SQSTM1/p62 (D5E2) Rabbit mAb

价格询价

品牌Cell Signaling Technology
货号
8025
产地USA
更新日期2026年05月28日

应用范围W, IP, IHC-P
适应物种H,Mk

美国CST中国公司(丁香通商铺)

品牌商

13 年钻石会员

详细信息
文献和实验
技术资料

抗体英文名：
SQSTM1/p62 (D5E2) Rabbit mAb
抗原：
synthetic peptide corresponding to residues surrounding Gly162 of human SQSTM1/p62 protein
应用范围：
W, IP, IHC-P
适应物种：
H,Mk
供应商：
CST
级别：
详见MSDS文件
保质期：
详见说明书
库存：
大量
是否单克隆：
1
保存条件：
-20°c
规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity

规格：		产品价格：	￥请询价
规格：	100 ul (10 western blots)	产品价格：	￥请询价
规格：	carrier free & custom formulation / quantity	产品价格：	￥请询价

pathway more info application references datasheet PDF MSDS PDF protocols

Applications Key: W=Western Blotting IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key: H=Human Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Applications	Reactivity	Sensitivity	MW (kDa)	Isotype
W IP IHC-P	H Mk	Endogenous	62	Rabbit IgG

Protocols	8025: IHC / Paraffin , Immunoprecipitation , Western Blotting
Specificity / Sensitivity	SQSTM1/p62 (D5E2) Rabbit mAb recognizes endogenous levels of total SQSTM1/p62 protein.
Source / Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly162 of human SQSTM1/p62 protein. Western Blotting Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence^® Control siRNA (Unconjugated) #6568 (-), SignalSilence^® SQSTM1/p62 siRNA I #6394 (+) or SignalSilence^® SQSTM1/p62 siRNA II #6399 (+), using SQSTM1/p62 (D5E2) Rabbit mAb (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The SQSTM1/p62 (D5E2) Rabbit mAb confirms silencing of SQSTM1/p62 expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control. Western Blotting Western blot analysis of extracts from various cell lines using SQSTM1/p62 (D5E2) Rabbit mAb. Western Blotting Western blot analysis of extracts from SK-MEL-2 cells, untreated (-) or starved overnight in Earle's Balanced Salt Solution (EBSS) (+), using SQSTM1/p62 (D5E2) Rabbit mAb. Western Blotting Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a tagged human SQSTM1/p62 construct (+), using SQSTM1/p62 (D5E2) Rabbit mAb. IHC-P (paraffin) Immunohistochemical analysis of paraffin-embedded human breast carcinoma using SQSTM1/p62 (D5E2) Rabbit mAb. IHC-P (paraffin) Immunohistochemical analysis of paraffin-embedded human colon carcinoma using SQSTM1/p62 (D5E2) Rabbit mAb.
Background	Sequestosome 1 (SQSTM1, p62) is a ubiquitin binding protein involved in cell signaling, oxidative stress, and autophagy (1-4). It was first identified as a protein that binds to the SH2 domain of p56Lck (5), and independently found to interact with PKCζ (6,7). It was subsequently found to interact with ubiquitin, providing a scaffold for several signaling proteins and triggering degradation of proteins through the proteasome or lysosome (8). Interaction between SQSTM1 and TRAF6 leads to the K63-linked polyubiquitination of TRAF6 and subsequent activation of the NF-κB pathway (9). Protein aggregates formed by SQSTM1 can be degraded by the autophagosome (4,10,11). SQSTM1 binds autophagosomal membrane protein LC3/Atg8, bringing SQSTM1-containing protein aggregates to the autophagosome (12). Lysosomal degradation of autophagosomes leads to a decrease in SQSTM1 levels during autophagy; conversely, autophagy inhibitors stabilize SQSTM1 levels. Studies have demonstrated a link between SQSTM1 and oxidative stress. SQSTM1 interacts with KEAP1, which is a cytoplasmic inhibitor of NRF2, a key transcription factor involved in cellular responses to oxidative stress (3). Thus, accumulation of SQSTM1 can lead to an increase in NRF2 activity. Kirkin, V. et al. (2009) Mol Cell 34, 259-69. Seibenhener, M.L. et al. (2007) FEBS Lett 581, 175-9. Komatsu, M. et al. (2010) Nat Cell Biol 12, 213-23. Bjørkøy, G. et al. (2006) Autophagy 2, 138-9. Joung, I. et al. (1996) Proc Natl Acad Sci USA 93, 5991-5. Sanchez, P. et al. (1998) Mol Cell Biol 18, 3069-80. Puls, A. et al. (1997) Proc Natl Acad Sci USA 94, 6191-6. Vadlamudi, R.K. et al. (1996) J Biol Chem 271, 20235-7. Wooten, M.W. et al. (2005) J Biol Chem 280, 35625-9. Bjørkøy, G. et al. (2005) J Cell Biol 171, 603-14. Komatsu, M. et al. (2007) Cell 131, 1149-63. Pankiv, S. et al. (2007) J Biol Chem 282, 24131-45.
Application References	Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !
Companion Products	5114 SQSTM1/p62 Antibody 7071 Phototope^® -HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody 7074 Anti-rabbit IgG, HRP-linked Antibody 7720 Prestained Protein Marker, Broad Range (Premixed Format) 7727 Biotinylated Protein Ladder Detection Pack 7003 20X LumiGLO^® Reagent and 20X Peroxide 8112 SignalStain^® Antibody Diluent 8114 SignalStain^® Boost IHC Detection Reagent (HRP, Rabbit) 5425 Normal Goat Serum 9997 Tris Buffered Saline with Tween 20 (TBST-10X) For Research Use Only. Not For Use In Diagnostic Procedures.