Description: Echelons Class III PI3K Activity Assay is a competitive ELISA in which the signal is inversely proportional to the amount of PI(3)P produced. After the PI3-K reactions are complete, reaction products are added to the PI(3)P-coated microplate, for competitive binding to a PI(3)P detector protein. The amount of PI(3)P detector protein bound to the plate is determined through colorimetric detection.