Phospho-Specific Polyclonal Antibody against p38 MAPK [Thr180/Tyr182]
Form
Affinity Purified Antibody
Host
Rabbit
Species Reactivity
p38 MAPK [Thr180/Tyr182] Polyclonal Antibody, Phospho-specific Human
[Ab]
0.1 mg/mL
Specificity
The p38 MAP kinase is a stress-activated protein kinase. The p38 MAP kinase is activated by treatment of cells with proinflammatory cytokines (e.g. TNF and IL-1) or by exposure of cells to environmental stress (e.g. UV radiation and osmotic shock). This activation results in the phosphorylation of residues Thr180 and Tyr182. This antibody is specific for the 39 kD p-38 protein phosphorylated at Thr180/Tyr182 It is purified by sequential chromatography on protein A and phospho- and dephospho- peptide affinity columns.
Uses
These antibodies are effective in immunoblotting, immunohistochemistry Immunoprecipitation, and ELISA.
Suggested Working Dilution
The optimal working dilution should be determined for each specific assay condition. This antibody is sold for laboratory research use only, not for human or in-vivo use. Covance antibodies may not be resold or modified for resale without prior written approval.
Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C.
Warranty/Conditions
Covance products may not be resold or modified for resale without prior written approval.