This antibody was raised against a synthetic phosphopeptide corresponding to amino acid residues surrounding the phospho Thr 202 and Tyr 204 of MAP kinase. It is specific for Mr 42/44 kD protein phosphorylated at Thr 202/Tyr204. Immunolabeling in UV treated Jurkat cells is blocked by the phosphopeptide but not by the dephosphopeptide. This antibody was purified by sequential chromatography on protein A and phospho- and dephospho- peptide affinity columns.
Uses
These antibodies are effective in immunoblotting.
Suggested Working Dilution
ERK (p44/42 MAP Kinase) [Thr202/Tyr204] Polyclonal Antibody, Phospho-Specific The optimal working dilution should be determined for each specific assay condition.
Western blot: 1:1,000
Storage
Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C.
Warranty/Conditions
Covance products may not be resold or modified for resale without prior written approval.