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- 询价记录
- 文献和实验
- 技术资料
- 保存条件:
-20度
- 保质期:
有效期1年
- 英文名:
Hieff Clone Zero TOPO-TA Cloning Kit
- 库存:
大量
- 供应商:
翌圣生物科技(上海)股份有限公司
- 规格:
20 T(10907ES20)/50 T(10907ES50)
| 规格: | 20 T(10907ES20) | 产品价格: | ¥408 |
|---|---|---|---|
| 规格: | 50 T(10907ES50) | 产品价格: | ¥1323 |
产品描述:
本试剂盒是利用拓扑异构酶(Topoisomerase)高效快速连接DNA片段的原理进一步研发而成,与传统的T4连接酶相比,具有以下优势:1)快速,仅需1-5分钟即可完成连接反应。2)高效,无自连现象,阳性克隆率接近100%,无需设置蓝白斑筛选;3)操作简单,从连接到涂板仅需15-20 min。操作过程省去冰浴、热激和1 h复苏等。4)可连接长达5 kb目的产物。
产品属性:
| 片段长度 | ≤5kb |
| 片段数量 | 1个 |
| 应用 | 分子克隆 |
| 片段类型 | A尾PCR产物 |
| 反应速度 | 5min |
产品特色:
- 快速,仅需1-5分钟即可完成连接反应
- 高效,无自连现象,阳性克隆率接近100%,无需设置蓝白斑筛选
- 操作简单,从连接到涂板仅需15-20 min。操作过程省去冰浴、热激和1 h复苏等
- 可连接长达5 kb目的产物
储存条件:
-25~-15℃保存,有效期1年。
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询价记录
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文献和实验[1] Qiu L, Wang Y, Tang W, et al. Activated Phosphoinositide 3-Kinase δ Syndrome: a Large Pediatric Cohort from a Single Center in China. J Clin Immunol. 2022;42(4):837-850. doi:10.1007/s10875-022-01218-4(IF:8.317)
[2] Gao R, Han T, Xun H, et al. MYB transcription factors GmMYBA2 and GmMYBR function in a feedback loop to control pigmentation of seed coat in soybean. J Exp Bot. 2021;72(12):4401-4418. doi:10.1093/jxb/erab152(IF:6.992)
[3] Liu Y, Lai M, Li S, et al. LS-106, a novel EGFR inhibitor targeting C797S, exhibits antitumor activities both in vitro and in vivo. Cancer Sci. 2022;113(2):709-720. doi:10.1111/cas.15229(IF:6.716)
[4] Yang Z, Li Y, Gao F, et al. MYB21 interacts with MYC2 to control the expression of terpene synthase genes in flowers of Freesia hybrida and Arabidopsis thaliana. J Exp Bot. 2020;71(14):4140-4158. doi:10.1093/jxb/eraa184(IF:5.908)
[5] Xu S, Mo C, Lin J, et al. Loss of ID3 drives papillary thyroid cancer metastasis by targeting E47-mediated epithelial to mesenchymal transition. Cell Death Discov. 2021;7(1):226. Published 2021 Aug 30. doi:10.1038/s41420-021-00614-w(IF:5.241)
[6] Zhu L, Xia C, Wu L, et al. The critical role of RasGRP4 in the growth of diffuse large B cell lymphoma. Cell Commun Signal. 2019;17(1):92. Published 2019 Aug 13. doi:10.1186/s12964-019-0415-6(IF:5.111)
[7] Huang JH, Jiao YH, Li L, Li DW, Li HY, Yang WD. Small RNA analysis of Perna viridis after exposure to Prorocentrum lima, a DSP toxins-producing dinoflagellate. Aquat Toxicol. 2021;239:105950. doi:10.1016/j.aquatox.2021.105950(IF:4.964)
[8] Li Y, Shan X, Gao R, et al. MYB repressors and MBW activation complex collaborate to fine-tune flower coloration in Freesia hybrida. Commun Biol. 2020;3(1):396. Published 2020 Jul 27. doi:10.1038/s42003-020-01134-6(IF:4.165)
[9] Li Y, Shan X, Tong L, et al. The Conserved and Particular Roles of the R2R3-MYB Regulator FhPAP1 from Freesia hybrida in Flower Anthocyanin Biosynthesis. Plant Cell Physiol. 2020;61(7):1365-1380. doi:10.1093/pcp/pcaa065(IF:4.062)
[10] Bao T, Shadrack K, Yang S, et al. Functional Characterization of Terpene Synthases Accounting for the Volatilized-Terpene Heterogeneity in Lathyrus odoratus Cultivar Flowers. Plant Cell Physiol. 2020;61(10):1733-1749. doi:10.1093/pcp/pcaa100(IF:4.062)
[11] Guo L, Zhang M, Gao Y, et al. A gonadal mosaicism novel KMT2D mutation identified by haplotype construction and clone sequencing strategy. Clin Chim Acta. 2022;531:197-203. doi:10.1016/j.cca.2022.04.008(IF:3.786)
[12] Liu Q, Zhang L, Shu Z, Ding Y, Tang XM, Zhao XD. Two paternal mosaicism of mutation in ELANE causing severe congenital neutropenia exhibit normal neutrophil morphology and ROS production. Clin Immunol. 2019;203:53-58. doi:10.1016/j.clim.2019.04.008(IF:3.548)
[13] Li GY, Liu XJ, Fang GQ, Yang J, Zhan S, Li MW. Functional characterization of a low-density lipoprotein receptor in the lepidopteran model, Bombyx mori [published online ahead of print, 2022 Apr 11]. Insect Sci. 2022;10.1111/1744-7917.13018. doi:10.1111/1744-7917.13018(IF:3.262)
[14] Chen L, Ren Y, Han K, Stoeck T, Jiang J, Pan H. Re-description and molecular phylogeny of the free-swimming peritrichs Hastatella radians Erlanger, 1890 and H. aesculacantha Jarocki & Jakubowska, 1927 (Ciliophora, Peritrichia) from China. Eur J Protistol. 2022;84:125891. doi:10.1016/j.ejop.2022.125891(IF:3.020)
[15] Zhuang L, Ji Y, Tian P, et al. Polymerase chain reaction combined with fluorescent lateral flow immunoassay based on magnetic purification for rapid detection of canine parvovirus 2. BMC Vet Res. 2019;15(1):30. Published 2019 Jan 17. doi:10.1186/s12917-019-1774-3(IF:1.792)
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