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Roche货号10481220001、10799009001
、10716359001现货T4 DNA连接酶19121878899上海睿安生物Merck-Sigma-Roche-Millipore官方正式授权代理商- ￥1247.84
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
T4 DNA
- 保质期：
1年
- 保存条件：
-20°C
- 库存：
30⁺瓶
- 供应商：
上海睿安生物19121878899
- 规格：
100 Units/瓶
Roche货号10481220001、10799009001、10716359001现货T4 DNA连接酶19121878899上海睿安生物Merck-Sigma-Roche-Millipore官方正式授权代理商
T4 DNA连接酶
solution,from Escherichia coli(NM 989),suitable for activity assay
别名:dna连接酶，t4
属性
biological source:Escherichia coli(NM 989)
recombinant:expressed in E. coli
description:recombinant form of the enzyme from T4 phage
form:solution
specific activity:~3000 units/mg protein
packaging:pkg of 100U(10481220001[1U/μl]),pkg of 500U(10716359001[1U/μl]),pkg of 500U(10799009001[5U/μl])
manufacturer/tradename:Roche
parameter:4-22°C optimum reaction temp.
technique(s): activity assay:suitable
optimum pH:7.2-7.8
storage temp:−20°C
说明
General description:T4 DNA连接酶催化双链DNA中邻近的3′-羟基-和5′-磷酸末端之间磷酸二酯键的形成。双链DNA中单链切口也被T4 DNA连接酶封闭。
成分:T4 DNA连接酶，包括含有ATP的╳10倍浓缩连接缓冲液。
Application:连接粘性和平端DNA片段。
Biochem/physiol Actions
热灭活:可以通过加热使酶失活(65℃，10分钟)。
Preparation Note
激活剂:Mg2+(10mM)，DTT
工作溶液:为了稀释酶，Roche建议使用含有缓冲液组分的缓冲液:20mM Tris-HCl，60mM KCl，1mM EDTA，5mM二硫赤藓糖醇，50％甘油(v/v)，pH 7.5(4℃)。
工作溶液:为了稀释酶，Roche建议使用含有缓冲液组分的缓冲液:20mM Tris-HCl，60mM KCl，1mM EDTA，5mM二硫赤藓糖醇，50％甘油(v/v)，pH 7.5(4℃)。
Analysis Note
质量
根据现行质量控制程序，测试不存在脱氧核糖核酸酶和核酸外切酶。
根据现行质量控制程序，测试不存在脱氧核糖核酸酶和核酸外切酶。
Other Notes
一个单位的T4 DNA连接酶是在+37°C下在20分钟内将1nmol 32P从焦磷酸盐转化为诺芮特可吸收材料的酶活性量。一个单位对应于在核酸外切酶III抗性测定中测定的0.2U。稀释缓冲液:50mM Tris-HCl，10mM二硫赤藓糖醇，牛血清白蛋白，500μg/ml，pH 7.6(+25°C)。
体积活性:1╳103U/ml；5╳103U/ml
体积活性:1╳103U/ml；5╳103U/ml
仅用于生命科学研究。不可用于诊断。
同行评审论文(部分:文献)
Use of a lentivirus/VSV pseudotype virus for highly efficient genetic redirection of human peripheral blood lymphocytes.
Anthony Simmons et al.
Nature protocols, 1(6), 2688-2700 (2007-04-05)
Genetic redirection of lymphocytes that have been engineered to recognize antigens other than those originally programmed by their rearranged germlines is a potentially powerful immunotherapeutic tool. The rationale for the protocol described here is that many cancers and persistent or
Autophagy activation clears ELAVL1/HuR-mediated accumulation of SQSTM1/p62 during proteasomal inhibition in human retinal pigment epithelial cells.
Johanna Viiri et al.
PloS one, 8(7), e69563-e69563 (2013-08-08)
Age-related macular degeneration (AMD) is the most common reason of visual impairment in the elderly in the Western countries. The degeneration of retinal pigment epithelial cells (RPE) causes secondarily adverse effects on neural retina leading to visual loss. The aging
'Calling Cards' method for high-throughput identification of targets of yeast DNA-binding proteins.
Haoyi Wang et al.
Nature protocols, 3(10), 1569-1577 (2008-09-20)
We present a protocol for a novel method for identifying the targets of DNA-binding proteins in the genome of the yeast Saccharomyces cerevisiae. This is accomplished by engineering a DNA-binding protein so that it leaves behind in the genome a
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文献和实验该产品被引用文献
Roche货号10481220001、10799009001、10716359001现货T4 DNA连接酶19121878899上海睿安生物Merck-Sigma-Roche-Millipore官方正式授权代理商
T4 DNA连接酶
solution,from Escherichia coli(NM 989),suitable for activity assay
别名:dna连接酶，t4
属性
biological source:Escherichia coli(NM 989)
recombinant:expressed in E. coli
description:recombinant form of the enzyme from T4 phage
form:solution
specific activity:~3000 units/mg protein
packaging:pkg of 100U(10481220001[1U/μl]),pkg of 500U(10716359001[1U/μl]),pkg of 500U(10799009001[5U/μl])
manufacturer/tradename:Roche
parameter:4-22°C optimum reaction temp.
technique(s): activity assay:suitable
optimum pH:7.2-7.8
storage temp:−20°C
说明
General description:T4 DNA连接酶催化双链DNA中邻近的3′-羟基-和5′-磷酸末端之间磷酸二酯键的形成。双链DNA中单链切口也被T4 DNA连接酶封闭。
成分:T4 DNA连接酶，包括含有ATP的╳10倍浓缩连接缓冲液。
Application:连接粘性和平端DNA片段。
Biochem/physiol Actions
热灭活:可以通过加热使酶失活(65℃，10分钟)。
Preparation Note
激活剂:Mg2+(10mM)，DTT
工作溶液:为了稀释酶，Roche建议使用含有缓冲液组分的缓冲液:20mM Tris-HCl，60mM KCl，1mM EDTA，5mM二硫赤藓糖醇，50％甘油(v/v)，pH 7.5(4℃)。
工作溶液:为了稀释酶，Roche建议使用含有缓冲液组分的缓冲液:20mM Tris-HCl，60mM KCl，1mM EDTA，5mM二硫赤藓糖醇，50％甘油(v/v)，pH 7.5(4℃)。
Analysis Note
质量
根据现行质量控制程序，测试不存在脱氧核糖核酸酶和核酸外切酶。
根据现行质量控制程序，测试不存在脱氧核糖核酸酶和核酸外切酶。
Other Notes
一个单位的T4 DNA连接酶是在+37°C下在20分钟内将1nmol 32P从焦磷酸盐转化为诺芮特可吸收材料的酶活性量。一个单位对应于在核酸外切酶III抗性测定中测定的0.2U。稀释缓冲液:50mM Tris-HCl，10mM二硫赤藓糖醇，牛血清白蛋白，500μg/ml，pH 7.6(+25°C)。
体积活性:1╳103U/ml；5╳103U/ml
体积活性:1╳103U/ml；5╳103U/ml
仅用于生命科学研究。不可用于诊断。
同行评审论文(部分:文献)
Use of a lentivirus/VSV pseudotype virus for highly efficient genetic redirection of human peripheral blood lymphocytes.
Anthony Simmons et al.
Nature protocols, 1(6), 2688-2700 (2007-04-05)
Genetic redirection of lymphocytes that have been engineered to recognize antigens other than those originally programmed by their rearranged germlines is a potentially powerful immunotherapeutic tool. The rationale for the protocol described here is that many cancers and persistent or
Autophagy activation clears ELAVL1/HuR-mediated accumulation of SQSTM1/p62 during proteasomal inhibition in human retinal pigment epithelial cells.
Johanna Viiri et al.
PloS one, 8(7), e69563-e69563 (2013-08-08)
Age-related macular degeneration (AMD) is the most common reason of visual impairment in the elderly in the Western countries. The degeneration of retinal pigment epithelial cells (RPE) causes secondarily adverse effects on neural retina leading to visual loss. The aging
'Calling Cards' method for high-throughput identification of targets of yeast DNA-binding proteins.
Haoyi Wang et al.
Nature protocols, 3(10), 1569-1577 (2008-09-20)
We present a protocol for a novel method for identifying the targets of DNA-binding proteins in the genome of the yeast Saccharomyces cerevisiae. This is accomplished by engineering a DNA-binding protein so that it leaves behind in the genome a
文献支持
Roche货号10481220001、10799009001、10716359001现货T4 DNA连接酶19121878899上海睿安生物Merck-Sigma-Roche-Millipore官方正式授权代理商
￥1247.84
