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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件:
-20℃保存,避免反复冻融
- 保质期:
有效期1年
- 英文名:
GST-tag,Mouse mAb
- 库存:
大量
- 供应商:
翌圣生物科技(上海)股份有限公司
- 规格:
10μl
| 产品名称 | 产品编号 | 规格 | 储存 | 价格(元) |
| GST-tag, Mouse mAb小鼠抗GST-tag单克隆抗体 | 30901ES10 | 10μl (>50次) | -20℃ | 218.00 |
| GST-tag, Mouse mAb小鼠抗GST-tag单克隆抗体 | 30901ES50 | 50μl (>250次) | -20℃ | 688.00 |
融合蛋白表达中通常在蛋白N-端或C-端加上特定蛋白或多肽序列作为一种抗原表位(标签蛋白)以便于目的蛋白后续的定位、表达、检测及纯化。Glutathione S-Transferase(即谷胱甘肽S-转移酶,GST)是应用非常广泛的一种蛋白标签,来源于Schistosomajaponicum,分子量为26kDa,可应用于各种融合蛋白,可在大肠杆菌和酵母菌等宿主细胞中表达,用GST亲和柱可简便地分离融合蛋白。 GST标签有助于保护重组蛋白免受胞外蛋白酶的降解并提高其稳定性。
GST抗体可以用于GST融合蛋白后续的表达、细胞内定位,纯化、以及定性或定量分析。
推荐稀释倍数
WB : 1:5000-1:50000
IP : 1:5000-1:50000
ELISA: 1:10000-1:50000
产品介绍
| 产品类型(Isotype) | 小鼠单抗(mouse McAb IgG2a) |
| 分子量(Molecular Weight) | 26 kDa |
| 反应性(Specificity) | Recombinant protein, Schistosomajaponicum |
| 应用(Application) | WB/IP/ELISA |
| 免疫原(Immunogen) | Recombinant Protein |
| 纯化方式(Purified) | Protein A purification |
| 储存缓冲液(Storage Buffer) | PBS with 0.02% sodium azide and 50% glycerol pH 7.3。 |
冰袋(wet ice)运输。-20℃保存,避免反复冻融。
注意事项
1)使用前请先离心,以免造成不必要的损失。
相关产品
| 产品编号 | 产品名称 | 规格 | 价格(元) |
| 30902ES20 | GST-tag, Rabbit pAb兔抗GST-tag多克隆抗体 | 20μl (>40次) | 218.00 |
| 30902ES60 | GST-tag, Rabbit pAb兔抗GST-tag多克隆抗体 | 100μl (>200次) | 868.00 |
| 30903ES10 | HRP-conjugated GST-tag, Mouse mAb小鼠抗GST-tag单克隆抗体,HRP标记 | 10μl (>50次) | 218.00 |
| 30903ES50 | HRP-conjugated GST-tag, Mouse mAb小鼠抗GST-tag单克隆抗体,HRP标记 | 50μl (>250次) | 688.00 |
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文献和实验[2] Kang Y, Wu JX, Chen L. Structure of voltage-modulated sodium-selective NALCN-FAM155A channel complex. Nat Commun. 2020;11(1):6199. Published 2020 Dec 3. doi:10.1038/s41467-020-20002-9(IF:12.121)
[3] Tang C, Wu X, Ren Q, Yao M, Xu S, Yan Z. Hedgehog signaling is controlled by Rac1 activity. Theranostics. 2022;12(3):1303-1320. Published 2022 Jan 1. doi:10.7150/thno.67702(IF:11.556)
[4] Han L, Zhang F, Liu Y, et al. Uterus globulin associated protein 1 (UGRP1) binds podoplanin (PDPN) to promote a novel inflammation pathway during Streptococcus pneumoniae infection. Clin Transl Med. 2022;12(6):e850. doi:10.1002/ctm2.850(IF:11.492)
[5] Ai H, Cao Y, Jain A, et al. The ferroxidase LPR5 functions in the maintenance of phosphate homeostasis and is required for normal growth and development of rice. J Exp Bot. 2020;71(16):4828-4842. doi:10.1093/jxb/eraa211(IF:5.908)
[6] Wu Q, Huang Y, Gu L, Chang Z, Li GM. OTUB1 stabilizes mismatch repair protein MSH2 by blocking ubiquitination. J Biol Chem. 2021;296:100466. doi:10.1016/j.jbc.2021.100466(IF:5.157)
[7] Wang D, Tan KS, Arias-Moreno X, Tan W, Cheng G. Sirt3 increases CNPase enzymatic activity through deacetylation and facilitating substrate accessibility. Biochem Biophys Res Commun. 2021;571:181-187. doi:10.1016/j.bbrc.2021.07.079(IF:3.575)
(adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
Expression of Full Length Monoclonal Antibodies (mAb) in Algal Chloroplast
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