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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保质期:
1年以上
- 英文名:
SMARTer RACE cDNA Amplification Kit(Clontech:634923)
- 库存:
超低温产品订货3-5天
- 供应商:
北京智杰方远
- 保存条件:
-70度冻存
- 规格:
10T
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文献和实验利用RACE [Rapid Amplification of cDNA ENDs,cDNA末端的快速扩增],可以很简单地得到mRNA的5'末端序列,然而5'RACE是一个很复杂的技术,它的成功依赖每一步反应的有效完成。cDNA第一链的合成是由一个反义的基因特异性引物(Gene-specific primer,GSP)来起始的,cDNA第一链纯化后,利用末端脱氧核苷酸转移酶(Terminal deoxynucleotidyl transferase,TdT)在cDNA的5'末端加上一个合成
Rapid Amplification of cDNA Ends (RACE)
Rapid Amplification of cDNA ends (RACE) provides an inexpensive and powerful tool to quickly obtain full-length cDNA when the sequence is only partially known. Starting with an mRNA mixture, gene-specific primers generated from the known
RACE System for Rapid Amplification of cDNA Ends
-specific primer (GSP2, designed by the user) that anneals to a site located within the cDNA molecule, and a novel deoxyinosine-containing anchor primer (patent pending) provided with the system. Following amplification, 5' RACE products can be cloned










