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- 详细信息
- 文献和实验
- 技术资料
- 保质期:
1年
- 英文名:
Lenti-X™ HTX Packaging System
- 供应商:
北京智杰方远
- 保存条件:
-20度
- 规格:
20 rxns
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文献和实验Creation and Use of Infectious Virus Vector
Creation and use of your infectious vector: Plate 5 x 105 293T cells in 6 cm2 dishes containing 5 ml of media. (This can be scaled up if desired). The following day set up (use polypropylene tubes
monoclonal antibody production fusion
), 3 L, 0.22 (or 0.45) µm-filtered, store at -20ѓC = Asp-Arg x100, 1 L + Gluta x 50, 2 L + mercaptoethanol, 350 µl Asp-Arg x100, 1 L, pH 6.0 = L-Asparagine, 3.6 g (0.24 mM) + L-Arginine (0.55 mM), 11.6 g + HCl, 0.5N, 100 ml -> heat to 37ѓC
Inducible shRNA Lentiviral Vectors
of your expression clone. 3) Cotransfect the ViraPower™ Packaging Mix and pLenti6/V5-GW/miR expression plasmid DNA into 293FT Cells using Lipofectamine™ 2000. 4) Harvest virus-containing supernatants 48-72 hours post-transfection. 4. Titering
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