- ￥3155.95
- Roche
- made in USA
- 11420429001
- 2025年09月21日
11 年金牌会员
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保质期：
1年
- 英文名：
lys-c，protease
- 库存：
100⁺支
- 供应商：
上海睿安生物13611631389
- 保存条件：
2-8°C
- 规格：
5ug/支
畅销供应Roche货号11420429001蛋白内切酶Lys-C测序级(规格5ug/支)(目录价¥3155.95元/支)13611631389上海睿安生物
畅销供应Roche货号11047825001蛋白内切酶Lys-C测序级[规格(3╳5ug)/支](目录价¥8468.15元/支)13611631389上海睿安生物
一般描述:内蛋白酶赖氨酸-碳测序等级是从产酶溶血杆菌中分离出来的，是一种高度纯化和特异的蛋白酶。
特异性:这种酶是一种丝氨酸蛋白酶，能特异性水解赖氨酸羧基侧的酰胺、酯和肽键(在三-盐酸缓冲液中pH 7.0至9.0)。
热失活:酶可以通过加热(100℃下5分钟)或TCA变性。
应用:使用内蛋白酶赖氨酸-碳测序等级进行蛋白质结构分析和序列分析。它适用于消化溶液、聚丙烯xī酰胺凝胶或吸水膜中的蛋白质。
制备说明:储存条件(工作溶液)+2至+8℃
重悬:冻干内蛋白酶赖氨酸-碳测序等级在50& #956；L双蒸水中重构。生成缓冲液浓度为50mM Hepes、pH 8.0、10mM乙二èr胺四乙酸和5mg/ml棉子糖。为避免自溶，培养温度不应超过37℃。在4M尿素中稳定。
其他说明:仅用于生命科学研究。不可用于诊断。
一、╳※试剂※╳:常规血清优势供应:VivaCell货号C04001-500血清、Gemini货号900-108血清、Gemini血清、Gibco血清(10099-141澳洲血清、10270-106南美血清、16000-044血清)、BI血清BI货号04-001-1ACS、Sigma货号F8318-500ml澳洲胎牛血清、Sigma货号F8687澳洲胎牛血清、Sigma货号F2442-500ml特级胎牛血清、ScienCell货号0500澳洲胎牛血清、Cegrogen货号A0500-3010胎牛血清、Cegrogen货号B0500-5010新生牛血清、康源血清
培养基(包括无血清)、冻存液(包括无血清)，包括:友康Yocon
Merck(Millipore/Sigma/Roche/KaPa/Supelco/Avanti/Corning/Brand)正式授权代理、博奥龙Biodragon独dú家总代理、EFL独dú家总代理、碧云天Beyotime代理
☆☆☆☆☆☆Beckman货号A63881纯化磁珠Agencourt AMPure XP(60ml/瓶)/A63882(450ml/瓶)；Beckman货号B23317核酸片段筛选试剂盒Agencourt SPRIselect(5ml/瓶)
分子类:Biotium货号41003的GelRed染料、货号31000的EvaGreen染料、Zymo Research、Qiagen
饲料类:Research Diets、Dyets
二、╳※耗材※╳:
洁特JET耗材(各类常规用实验耗材)、赛普Cellpro耗材/KirGen耗材、博麦迪(常规的)各类试剂瓶
Bioplastics
Thermo Fisher Scientific的很多产品线，包括旗下的:
一、Nunc耗材
二、QSP耗材(各类吸头/滤芯吸头、离心管、PCR管等)
三、Nalgene耗材仪器
四、FisherBrand耗材仪器(含各类盖玻片载玻片等)
五、Lab_Serv耗材仪器
六、Finnpipette移液器(如：F1系列、F2系列、F3系列等等各类型号的移液器)
七、Abgene耗材
畅销款:我们是常备有大量现货的
同行评审论文
Enzymatic generation of peptides flanked by basic amino acids to obtain MS/MS spectra with 2× sequence coverage.
H Alexander Ebhardt et al.
Rapid communications in mass spectrometry : RCM, 28(24), 2735-2743 (2014-11-08)
Tandem mass (MS/MS) spectra generated by collision-induced dissociation (CID) typically lack redundant peptide sequence information in the form of e.g. b- and y-ion series due to frequent use of sequence-specific endopeptidases cleaving C- or N-terminal to Arg or Lys residues.
Heterologous expression and pro-peptide supported refolding of the high specific endopeptidase Lys-C
Stressler T, et al.
Protein Expression and Purification, 118, 31-38 (2016)
A bioinformatics approach for biomarker identification in radiation-induced lung inflammation from limited proteomics data.
Jung Hun Oh et al.
Journal of proteome research, 10(3), 1406-1415 (2011-01-14)
Many efforts have been made to discover novel bio-markers for early disease detection in oncology. However, the lack of efficient computational strategies impedes the discovery of disease-specific biomarkers for better understanding and management of treatment outcomes. In this study, we
Identification of N-terminally arginylated proteins and peptides by mass spectrometry.
Tao Xu et al.
Nature protocols, 4(3), 325-332 (2009-02-21)
This protocol outlines the essential steps of mass spectrometry-based analysis of protein samples that can be used to identify post-translational arginylation. We describe special considerations for sample preparation and digestion, mass spectrometry analysis using high-precision instruments, database searching for the
Alterations in oxidative phosphorylation complex proteins in the hearts of transgenic mice that overexpress the p38 MAP kinase activator, MAP kinase kinase 6.
Jason A Wall et al.
American journal of physiology. Heart and circulatory physiology, 291(5), H2462-H2472 (2006-06-13)
Ischemia-reperfusion (I/R) has critical consequences in the heart. Recent studies on the functions of I/R-activated kinases, such as p38 mitogen-activated protein kinase (MAPK), showed that I/R injury is reduced in the hearts of transgenic mice that overexpress the p38 MAPK
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
- 作者
- 内容
- 询问日期
文献和实验该产品被引用文献
畅销供应Roche货号11420429001蛋白内切酶Lys-C测序级(规格5ug/支)(目录价¥3155.95元/支)13611631389上海睿安生物
畅销供应Roche货号11047825001蛋白内切酶Lys-C测序级[规格(3╳5ug)/支](目录价¥8468.15元/支)13611631389上海睿安生物
一般描述:内蛋白酶赖氨酸-碳测序等级是从产酶溶血杆菌中分离出来的，是一种高度纯化和特异的蛋白酶。
特异性:这种酶是一种丝氨酸蛋白酶，能特异性水解赖氨酸羧基侧的酰胺、酯和肽键(在三-盐酸缓冲液中pH 7.0至9.0)。
热失活:酶可以通过加热(100℃下5分钟)或TCA变性。
应用:使用内蛋白酶赖氨酸-碳测序等级进行蛋白质结构分析和序列分析。它适用于消化溶液、聚丙烯xī酰胺凝胶或吸水膜中的蛋白质。
制备说明:储存条件(工作溶液)+2至+8℃
重悬:冻干内蛋白酶赖氨酸-碳测序等级在50& #956；L双蒸水中重构。生成缓冲液浓度为50mM Hepes、pH 8.0、10mM乙二èr胺四乙酸和5mg/ml棉子糖。为避免自溶，培养温度不应超过37℃。在4M尿素中稳定。
其他说明:仅用于生命科学研究。不可用于诊断。
一、╳※试剂※╳:常规血清优势供应:VivaCell货号C04001-500血清、Gemini货号900-108血清、Gemini血清、Gibco血清(10099-141澳洲血清、10270-106南美血清、16000-044血清)、BI血清BI货号04-001-1ACS、Sigma货号F8318-500ml澳洲胎牛血清、Sigma货号F8687澳洲胎牛血清、Sigma货号F2442-500ml特级胎牛血清、ScienCell货号0500澳洲胎牛血清、Cegrogen货号A0500-3010胎牛血清、Cegrogen货号B0500-5010新生牛血清、康源血清
培养基(包括无血清)、冻存液(包括无血清)，包括:友康Yocon
Merck(Millipore/Sigma/Roche/KaPa/Supelco/Avanti/Corning/Brand)正式授权代理、博奥龙Biodragon独dú家总代理、EFL独dú家总代理、碧云天Beyotime代理
☆☆☆☆☆☆Beckman货号A63881纯化磁珠Agencourt AMPure XP(60ml/瓶)/A63882(450ml/瓶)；Beckman货号B23317核酸片段筛选试剂盒Agencourt SPRIselect(5ml/瓶)
分子类:Biotium货号41003的GelRed染料、货号31000的EvaGreen染料、Zymo Research、Qiagen
饲料类:Research Diets、Dyets
二、╳※耗材※╳:
洁特JET耗材(各类常规用实验耗材)、赛普Cellpro耗材/KirGen耗材、博麦迪(常规的)各类试剂瓶
Bioplastics
Thermo Fisher Scientific的很多产品线，包括旗下的:
一、Nunc耗材
二、QSP耗材(各类吸头/滤芯吸头、离心管、PCR管等)
三、Nalgene耗材仪器
四、FisherBrand耗材仪器(含各类盖玻片载玻片等)
五、Lab_Serv耗材仪器
六、Finnpipette移液器(如：F1系列、F2系列、F3系列等等各类型号的移液器)
七、Abgene耗材
畅销款:我们是常备有大量现货的
同行评审论文
Enzymatic generation of peptides flanked by basic amino acids to obtain MS/MS spectra with 2× sequence coverage.
H Alexander Ebhardt et al.
Rapid communications in mass spectrometry : RCM, 28(24), 2735-2743 (2014-11-08)
Tandem mass (MS/MS) spectra generated by collision-induced dissociation (CID) typically lack redundant peptide sequence information in the form of e.g. b- and y-ion series due to frequent use of sequence-specific endopeptidases cleaving C- or N-terminal to Arg or Lys residues.
Heterologous expression and pro-peptide supported refolding of the high specific endopeptidase Lys-C
Stressler T, et al.
Protein Expression and Purification, 118, 31-38 (2016)
A bioinformatics approach for biomarker identification in radiation-induced lung inflammation from limited proteomics data.
Jung Hun Oh et al.
Journal of proteome research, 10(3), 1406-1415 (2011-01-14)
Many efforts have been made to discover novel bio-markers for early disease detection in oncology. However, the lack of efficient computational strategies impedes the discovery of disease-specific biomarkers for better understanding and management of treatment outcomes. In this study, we
Identification of N-terminally arginylated proteins and peptides by mass spectrometry.
Tao Xu et al.
Nature protocols, 4(3), 325-332 (2009-02-21)
This protocol outlines the essential steps of mass spectrometry-based analysis of protein samples that can be used to identify post-translational arginylation. We describe special considerations for sample preparation and digestion, mass spectrometry analysis using high-precision instruments, database searching for the
Alterations in oxidative phosphorylation complex proteins in the hearts of transgenic mice that overexpress the p38 MAP kinase activator, MAP kinase kinase 6.
Jason A Wall et al.
American journal of physiology. Heart and circulatory physiology, 291(5), H2462-H2472 (2006-06-13)
Ischemia-reperfusion (I/R) has critical consequences in the heart. Recent studies on the functions of I/R-activated kinases, such as p38 mitogen-activated protein kinase (MAPK), showed that I/R injury is reduced in the hearts of transgenic mice that overexpress the p38 MAPK
技术资料需要更多技术资料 索取更多技术资料
资料下载：
西格玛奥德里奇&睿安2022授权书.pdf 附 (下载 0 次)
默克化工&睿安2022授权书.pdf 附 (下载 0 次)
西格玛奥德里奇&睿安2022授权书.png 附 (下载 0 次)
默克化工&睿安2022授权书.png 附 (下载 0 次)
请 [登录] 后再下载!
文献支持
Roche货号11420429001蛋白内切酶Lys-C测序级13611631389上海睿安生物
￥3155.95
