血清和血浆miRNA PCR芯片 Serum & Plasm

Human Serum & Plasma miRNA PCR Array: MIHS-106Z

The Human Serum & Plasma miScript miRNA PCR Array profiles the expression of 84 miRNAs detectable and differentially expressed in serum, plasma, and other bodily fluids. This array provides disease and toxicology researchers with a convenient way to quickly analyze the miRNAs most relevant to pathophysiological conditions. These miRNAs have been carefully selected based on results published in peer-reviewed journals that suggest a correlation with serum expression levels and specific diseases. The discovery of these master regulatory molecules in serum and biological fluids, and their apparently regulated levels there, suggest a mechanistic role for miRNA in both normal and pathophysiological processes. Their presence in noninvasive, easily accessible samples provides readily available source material for research purposes. The profiling results from this array can potentially serve as a useful molecular marker for heart and liver injury or disease, atherosclerosis, diabetes, and a number of organ-specific cancers. Other miRNAs routinely present in serum are included to serve as positive controls for the successful detection of serum miRNA expression. This array can also be used with miRNeasy Serum/Plasma Spike-In Control (catalog number 219610). When spiked into the sample before nucleic acid isolation and detected with the control assay, it provides a more useful normalization method for this application than the typical reference short noncoding RNAs not normally detected in serum. A set of controls present on this array enables data analysis using the ΔΔCT method of relative quantification, assessment of reverse transcription performance, and assessment of PCR performance. Using SYBR Green-based real-time PCR, the expression of a focused panel of miRNAs found in serum, plasma, and other bodily fluids can be easily and reliably analyzed with this miScript miRNA PCR Array. miScript miRNA PCR Arrays are intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease. 96-well, 384-well ( 4 x 96 ), and Rotor-Disc 100 formats are available.

Injury:
Heart Injury: miR-1, miR-133a, miR-208a, miR-423-5p, miR-499a-5p.
Liver Injury: miR-122-5p, miR-192-5p.
Disease:
Atherosclerosis: miR-150-5p.
Diabetes: miR-124-3p, miR-146a-5p, miR-29a-3p, miR-30d-5p, miR-34a-5p, miR-375, miR-9-5p.
Heart Disease: miR-133a, miR-208a.
Liver Disease: miR-146a-5p, miR-215, miR-224-5p, miR-574-3p, miR-885-5p, miR-92a-3p.
Cancer:
Adenocarcinoma: miR-29a-3p, miR-92a-3p.
Breast: let-7a-5p, miR-10b-5p, miR-141-3p, miR-155-5p, miR-195-5p, miR-21-5p, miR-34a-5p.
Colon: miR-134, miR-146a-5p, miR-17-3p, miR-221-3p, miR-222-3p, miR-23a-3p, miR-29a-3p, miR-92a-3p.
Gastric: let-7a-5p, miR-1, miR-106b-5p, miR-17-5p, miR-17-3p, miR-20a-5p, miR-21-5p, miR-27a-3p, miR-34a-5p, miR-423-5p.
Leukemia & B Cell Lymphoma: miR-155-5p, miR-21-5p, miR-210.
Liver: miR-122-5p, miR-21-5p, miR-223-3p.
Lung: miR-134, miR-146a-5p, miR-17-3p, miR-21-5p, miR-210, miR-221-3p, miR-222-3p, miR-223-3p, miR-23a-3p, miR-25-3p.
Ovarian: miR-126-3p, miR-141-3p, miR-200a-3p, miR-200b-3p, miR-200c-3p, miR-203a, miR-205-5p, miR-21-5p, miR-214-3p, miR-29a-3p, miR-92a-3p, miR-93-5p.
Pancreatic: miR-196a-5p, miR-200a-3p, miR-200b-3p, miR-21-5p, miR-210.
Prostate: miR-100-5p, miR-125b-5p, miR-141-3p, miR-143-3p, miR-18a-5p, miR-19a-3p, miR-19b-3p, miR-20a-5p, miR-21-5p, miR-296-5p, miR-375.
Renal: miR-124-3p.
Rhabdomyosarcoma: miR-1, miR-133a, miR-133b.

Other Cancer-Related miRNA: let-7c, miR-107, miR-10a-5p, miR-128, miR-130b-3p, miR-145-5p, miR-148a-3p, miR-15a-5p, miR-184, miR-193a-5p, miR-204-5p, miR-206, miR-211-5p, miR-26b-5p, miR-30e-5p, miR-31-5p, miR-372, miR-373-3p, miR-374a-5p, miR-376c-3p, miR-7-5p, miR-96-5p.
Routinely Detectable in Normal Serum: miR-103a-3p, miR-15b-5p, miR-16-5p, miR-191-5p, miR-22-3p, miR-24-3p, miR-26a-5p.