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- Serum & Plasma 384HC
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- 2026年01月08日
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Serum & Plasma 384HC
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SAB
Human Serum & Plasma 384HC miRNA PCR Array: MIHS-3106Z
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The Human Serum & Plasma 384HC miScript miRNA PCR Array profiles the expression of 372 miRNAs detectable in serum and plasma using the miScript PCR System. This high-content array also includes the 84 miRNAs detectable and differentially expressed in serum, plasma, and other bodily fluids that are found on the Human Serum & Plasma miScript miRNA PCR Array. The serum and plasma 384HC array provides disease and toxicology researchers with a convenient way to quickly analyze the extracellular, circulating miRNAs most relevant to pathophysiological conditions. The discovery of miRNAs in serum and plasma, and their apparently regulated levels there, suggest a mechanistic role for these master regulatory molecules in both normal and pathophysiological processes. Their presence in serum and plasma samples provides readily available source material for research purposes. The profiling results from this array can potentially serve as a useful molecular marker for heart and liver injury or disease, atherosclerosis, diabetes, and a number of organ-specific cancers. This array can also be used with miRNeasy Serum/Plasma Spike-In Control (catalog number 219610). When spiked into the sample before nucleic acid isolation and detected with the control assay, it provides a more useful normalization method for this application than the typical reference short noncoding RNAs, which are not normally detected in serum. A set of controls present on this array enables data analysis using the ΔΔCT method of relative quantification, assessment of reverse transcription performance, and assessment of PCR performance. Using SYBR Green-based real-time PCR, the expression of a focused panel of miRNAs found in serum and plasma can be easily and reliably analyzed with this miScript miRNA PCR Array. miScript miRNA PCR Arrays are intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease |
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文献和实验和心肌梗塞(myocardial infraction)相关，miR-122 和药物造成的肝脏损伤有关1。 血清中 miRNA 抽提的挑战 1. 检体的备制：血清(serum)好还是血浆(plasma)好? 临床上会根据检验的标的来选择血液检体的保存及制备方式，血清和血浆都是来自血液检体的成分，但是根据制备的方式导致本质上含有成分有所不同。基于制备的方法可知，血清是血液经由凝血反应后，再经由离心沈淀所得到的上清液。而血浆则是血液在不凝血的状况下，经由离心去除血球后的液体
Screening for miRNA Expression Changes Using Quantitative PCR (Q-PCR)
In this section, we describe the use of Applied Biosystems TaqMan Array microRNA Card Set 3.0 to identify miRNA expression in a given RNA sample. This array set includes an array “A” and an array “B” which each have 384 wells that contain
microRNA芯片应用：筛选血清血浆中肿瘤早期诊断生物标志物
microRNA是一类约22nt的小分子RNA，调控基因表达。目前有研究显示，microRNA能以沉默复合体的形式稳定存在于人类的血清血浆中。癌症患者由于细胞破损，会释放一部分癌症特异的microRNA进入循环系统中，这部分microRNA就能作为生物标志物进行肿瘤的早期诊断。 安捷伦是国际领先的生物芯片制造企业。它生产的microRNA芯片通过独特的探针设计能区分成熟miRNA和miRNA前体，同时具有特异性高、灵敏度高等特点。特别适用于血清血浆microRNA的检测。
