心血管疾病miRNA PCR 芯片Cardiovascula

Human Cardiovascular Disease miRNA PCR Array: MIHS-113Z
The Human Cardiovascular Disease miScript miRNA PCR Array profiles the expression of 84 miRNAs known to exhibit altered expression during cardiovascular disease and development. This array provides researchers with a convenient way to quickly analyze the miRNAs most relevant to heart disease. Recent studies have established that miRNAs are crucial for the development and proper functioning of the heart. The heart expresses unique miRNAs regulated by cardiogenic transcription factors. During cardiovascular disease, the normal pattern of miRNA expression changes significantly and distinct patterns of miRNA expression appear to correlate with specific cardiovascular disorders. Deregulation of miRNA expression has been demonstrated in acute myocardial infarction (AMI or heart attack), cardiac hypertrophy, and cardiomyopathies, signifying the important role of miRNAs in heart disease. This array profiles miRNAs that are differentially regulated during cardiovascular disease, as identified by microarray, real-time RT-PCR, and deep sequencing expression profiling studies involving a broad spectrum of cardiovascular disease model systems, clinical samples, and cell lines. The profiling results from this array may yield insights into the molecular mechanisms behind the pathogenesis of heart disease. A set of controls present on this array enables data analysis using the ΔΔCT method of relative quantification, assessment of reverse transcription performance, and assessment of PCR performance. Using SYBR Green real-time PCR, the expression of a focused panel of miRNAs related to cardiovascular disease can be easily and reliably analyzed with this miScript miRNA PCR Array. miScript miRNA PCR Arrays are intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease. 96-well, 384-well ( 4 x 96 ), and Rotor-Disc 100 formats are available.

Myocardial Infarction:
Up-Regulated: let-7e-5p, miR-122-5p, miR-126-5p, miR-133b, miR-145-5p, miR-146a-5p, miR-15b-5p, miR-208a, miR-208b, miR-223-3p, miR-320a, miR-499a-5p.
Down-Regulated: miR-1, miR-107, miR-130a-3p miR-133a, miR-143-3p, miR-155-5p, miR-16-5p, miR-195-5p, miR-21-5p, miR-214-3p, miR-22-3p, miR-24-3p, miR-26a-5p, miR-26b-5p, miR-494.
Cardiac Hypertrophy:
Up-Regulated: let-7b-5p, let-7c, miR-103a-3p miR-125b-5p, miR-140-5p, miR-142-3p, miR-146a-5p, miR-18b-5p, miR-195-5p, miR-199a-5p, miR-208a, miR-208b, miR-21-5p, miR-214-3p, miR-221-3p, miR-222-3p, miR-224-5p, miR-23a-3p, miR-23b-3p, miR-24-3p, miR-25-3p, miR-27a-3p, miR-27b-3p, miR-31-5p, miR-424-5p.
Down-Regulated: miR-1, miR-126-5p, miR-133a, miR-133b, miR-149-5p, miR-150-5p, miR-181b-5p, miR-185-5p, miR-29a-3p, miR-29b-3p, miR-29c-3p, miR-30e-5p, miR-451a, miR-486-5p, miR-93-5p.
Regulated: miR-182-5p.
Cardiomyopathy:
Up-Regulated: let-7c, miR-100-5p, miR-103a-3p, miR-10b-5p, miR-125b-5p, miR-140-5p, miR-145-5p, miR-146a-5p, miR-181b-5p, miR-195-5p, miR-208a, miR-208b, miR-21-5p, miR-210, miR-214-3p, miR-221-3p, miR-222-3p, miR-23a-3p, miR-328, miR-342-3p, miR-423-3p, miR-499a-5p.
Down-Regulated: miR-1, miR-125a-5p, miR-126-5p, miR-133a, miR-133b, miR-143-3p, miR-29b-3p, miR-365a-3p, miR-378a-3p, miR-7-5p, miR-92a-3p.
Differentiation/Development:
Up-Regulated: let-7a-5p, let-7b-5p, let-7c, let-7d-5p, let-7f-5p, miR-1, miR-133a, miR-143-3p, miR-144-3p, miR-145-5p, miR-17-5p, miR-181a-5p, miR-206, miR-208a, miR-21-5p, miR-24-3p, miR-26a-5p, miR-27a-3p, miR-27b-3p, miR-30a-5p, miR-30c-5p, miR-30d-5p, miR-378a-3p, miR-93-5p, miR-98-5p, miR-99a-5p.
Down-Regulated: miR-124-3p, miR-125b-5p, miR-183-5p, miR-302a-3p, miR-302b-3p