通用DNA/siRNA转染试剂jetPRIME

通用DNA/siRNA转染试剂jetPRIME
高DNA转染效率

低量核酸

优越的细胞活力

一种用于DNA和/或siRNA转染的试剂

性价比高的
分子传递DNA、siRNA、DNA和siRNA
质粒转染

基因组编辑

病毒产生

siRNA转染

不同核酸的共传递
在血清存在下生长的贴壁细胞系

转染次数

1.5 ml JetTime®转染试剂足以在24孔板中进行1500次转染或在6孔板中进行375次转染
JetTime®是用于日常实验的强大转染试剂。它导致不同来源的转染粘附细胞系以及原代细胞的比例异常高。

与几种常用细胞系的best竞争对手试剂相比，JetTime®试剂的转染效率在70%到90%之间（图1和图2）。

Fig. 1: Comparative transfection efficiency of jetPRIME versus its main competitor. Transfection efficiency was assessed by FACS analysis in various cell lines 24 h after transfection in 24-well plates. Conditions were used according to the manufacturer’s recommendation for both Lipofectamine^® 2000 and for jetPRIME^®.

Fig. 2: Comparative transfection efficiency of jetPRIME^® versus Lipofectamine^® 3000. Transfection efficiency was assessed by FACS analysis in various cell lines 24 h after transfection in 96-well plates or 24-well plates. Conditions were used according to the manufacturer’s recommendation for lipofectamine^® 3000 and for jetPRIME^®.

Cost-effective: less DNA and less reagent

jetPRIME^® is a powerful in vitro transfection reagent that requires a small amount of reagent and plasmid DNA, making its use very cost-effective (Table 1).

Table 1: Recommended conditions to use. Amounts of DNA and reagent (jetPRIME^® and competitors) per well in 6-well plate for transfection according to manufacturers’ recommendations.

In addition to reducing costs, using less DNA also minimizes adverse cytotoxic effects triggered by transfection. Hence, jetPRIME^® is the reagent of choice for high transfection efficiency with excellent cell viability.

Better cell viability

jetPRIME^® is extremely gentle on cells during transfection leading to increased cell viability and improved transfection results. Cells transfected with jetPRIME^® are healthy, while major cytotoxicity is observed with competitors (Fig.3).

Fig. 3: Cell viability 24h after transfection. Phase contrast microscopy of HeLa cells 24 h after transfections performed according to the manufacturer’s recommendations for each reagent.

Plasmid transfection

jetPRIME^® leads to remarkably high percentages of transfected adherent cell lines of various origin, as well as primary cells (Table 1).

Table 1: Transfection efficiency of various cell types using jetPRIME^®. The percentage of GFP-positive cells was determined by FACS analysis 24 h after transfection.

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与其他试剂相比，JetTime的转染效率更高，细胞死亡也更少。细胞似乎不介意用转染试剂处理，反正也不需要改变培养基。我已经订购了更大容量的JetTime，并且已经使用了一半！可能很快会再订购一些！-