- ￥4551
- 法国Polyplus Transfection
- 101000053-Polyplus Transfection
- DNA转染试剂jetPEI
- 2025年07月14日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 供应商：
DNA转染试剂jetPEI
- 库存：
jetPEI
- 英文名：
jetPEI
- 保质期：
jetPEI
- 保存条件：
101000053
- 规格：
1ml
货号;101000053
规格;jetPEI® 1 mL + NaCl Solution 50 mL
非常适合贴壁细胞和悬浮细胞
HTS细胞转染的快速有效方法
与血清和抗生素兼容
异常可重复的结果
The batch protocol has been developed to prepare a homogeneous pool of transfected cells. For this purpose, the cells are transfected just after trypsinization, while still in suspension. This protocol is preferred for drug screening applications and allows rapid processing, one day faster than the forward protocol.
Fig. 1: jetPEI® reverse transfection protocol for HTS application.
| Reagent | jetPEI® |
|---|---|
| Molecule delivered | DNA |
| Applications | Plasmid transfection |
| Cell types | Adherent and suspension cells |
| Number of transfections | 1 ml of jetPEI® is sufficient to perform up to 2000 transfections in 96-well plates. |
| Storage | Store jetPEI® at 5 °C ± 3°C. |
| Provided with | 101-01N, 101-10N, 101-40N and 101B-010N are provided with 150 mM NaCl solution for complex formation |
| Reference Number | Amount of reagent | Amount of NaCl |
|---|---|---|
| 101000053 | jetPEI® 1 mL | 50 ml |
| 101000020 | jetPEI® 4 x 1 mL | 4 x 50 ml |
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- 作者
- 内容
- 询问日期
文献和实验| Cell Line | in vitro in vivo | Delivered Molecule | Reagent | Results & Citations | |
|---|---|---|---|---|---|
| HUVEC | in vitro | DNA | jetPEI |
Yoshizumi, M. et al. (2004)
Exp Cell Res 292, 1-10 Ebselen inhibits tumor necrosis factor-alpha-induced c-Jun N-terminal kinase activation and adhesion molecule expression in endothelial cells |
| HEK-293T | in vitro | DNA | jetPEI |
Cho, C. P. et al. (2013)
PLoS One 8, e62283 Regulation of programmed ribosomal frameshifting by co-translational refolding RNA hairpins | |
| HEK-293, HeLa, MCF7, MEF90 | in vitro | DNA | jetPEI |
Zhao, L. J. et al. (2009)
BMC Mol Biol 10, 89 Interaction of ZEB and histone deacetylase with the PLDLS-binding cleft region of monomeric C-terminal binding protein 2 | |
| HEK-293 | in vitro | DNA | jetPEI |
Condliffe, S. B. et al. (2013)
J Biol Chem 288, 33873-83 The E1015K variant in the synprint region of the CaV2.1 channel alters channel function and is associated with different migraine phenotypes | |
| HEK-293T | in vitro | DNA | jetPEI |
de Chassey, B. et al. (2013)
EMBO Rep 14, 938-44 Structure homology and interaction redundancy for discovering virus-host protein interactions | |
| BG1, HeLa | in vitro | DNA, siRNA | INTERFERin, jetPEI |
Docquier, A. et al. (2013)
Mol Endocrinol 27, 1429-41 Negative regulation of estrogen signaling by ERbeta and RIP140 in ovarian cancer cells | |
| HeLa | in vitro | DNA | jetPEI |
Feng, H. et al. (2013)
Cell Death Dis 4, e870 Calumenin-15 facilitates filopodia formation by promoting TGF-beta superfamily cytokine GDF-15 transcription | |
| HeLa | in vitro | DNA | jetPEI |
Germain, M. A. et al. (2013)
Mol Cell Proteomics , Elucidating Novel Hepatitis C Virus/Host Interactions Using Combined Mass Spectrometry and Functional Genomics Approaches. | |
| C2C12, HCT 116, MCF7 | in vitro | DNA, siRNA | jetPEI |
Guillaume, E. et al. (2013)
J Cell Sci , Flotillin micro-domains stabilize Cadherins at cell-cell junctions | |
| MIN6 | in vitro | DNA | jetPEI |
Hofmeister-Brix, A. et al. (2013)
Biochem J 456, 173-84 The ubiquitin-proteasome system regulates the stability and activity of the glucose sensor glucokinase in pancreatic beta-cells |
相关专题 总有一种转染方法适合你 近日，罗氏公司新推出了两款高性能的DNA转染试剂，为其细胞分析产品线再添新军。这两款名为X-tremeGENE 9和X-tremeGENE HP的转染试剂沿用了X-tremeGENE siRNA 转染试剂的前缀，应该与大名鼎鼎的Fugene 6不属于一类。据罗氏介绍，这两个产品是多组分的独特试剂，并非脂质体。它们能够提供高的转染效率、重复性和细胞存活率。 这两款新产品都融合了罗氏二十
不知道那些大侠做过,可以一起讨论一下.我试了几种转染试剂 ( JetpET, Lipofectine), 转染效率都极低。 只有电转的时候,转染效率也至多达到5%.不知道怎么拿这个细胞做luciferase.筛选浓度我已经用到1mg/ml了,不知道大家都用的多少?常规用FUGEGE6效果好点, 想效率高用逆转录病毒!"筛选浓度"你指什么? G418 我用过1200ug/ml, 1500ug/ml.对，就是指的G418.大侠你用过Fugene6吗?转染效率有多少?我一般都用FUGENE6, 不知
对于试验成功与否至关重要。最关键的是，应使用专门配制、输送小分子 RNA 的转染试剂（大部分市售的转染试剂设计用于大型质粒 DNA，而不是小 RNA 分子）。此外，仅有部分试剂设计用于特定细胞系的转染，而多数试剂的特异性范围较广。Lipofectamine RNAiMAX 是专为将 siRNAs 高效转染真核细胞而设计的转染试剂。 8.使用一个适当的阳性对照来优化转染和检测条件 对于大部分细胞，管家基因适合作为阳性对照。向靶标细胞转染几个浓度的、对您所选择的阳性对照特异的 siRNA。（这也适用
