- ￥4551
- 法国Polyplus Transfection
- 101000053-Polyplus Transfection
- DNA转染试剂jetPEI
- 2025年07月14日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 供应商：
DNA转染试剂jetPEI
- 库存：
jetPEI
- 英文名：
jetPEI
- 保质期：
jetPEI
- 保存条件：
101000053
- 规格：
1ml
货号;101000053
规格;jetPEI® 1 mL + NaCl Solution 50 mL
非常适合贴壁细胞和悬浮细胞
HTS细胞转染的快速有效方法
与血清和抗生素兼容
异常可重复的结果
The batch protocol has been developed to prepare a homogeneous pool of transfected cells. For this purpose, the cells are transfected just after trypsinization, while still in suspension. This protocol is preferred for drug screening applications and allows rapid processing, one day faster than the forward protocol.
Fig. 1: jetPEI® reverse transfection protocol for HTS application.
| Reagent | jetPEI® |
|---|---|
| Molecule delivered | DNA |
| Applications | Plasmid transfection |
| Cell types | Adherent and suspension cells |
| Number of transfections | 1 ml of jetPEI® is sufficient to perform up to 2000 transfections in 96-well plates. |
| Storage | Store jetPEI® at 5 °C ± 3°C. |
| Provided with | 101-01N, 101-10N, 101-40N and 101B-010N are provided with 150 mM NaCl solution for complex formation |
| Reference Number | Amount of reagent | Amount of NaCl |
|---|---|---|
| 101000053 | jetPEI® 1 mL | 50 ml |
| 101000020 | jetPEI® 4 x 1 mL | 4 x 50 ml |
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- 作者
- 内容
- 询问日期
文献和实验| Cell Line | in vitro in vivo | Delivered Molecule | Reagent | Results & Citations | |
|---|---|---|---|---|---|
| HUVEC | in vitro | DNA | jetPEI |
Yoshizumi, M. et al. (2004)
Exp Cell Res 292, 1-10 Ebselen inhibits tumor necrosis factor-alpha-induced c-Jun N-terminal kinase activation and adhesion molecule expression in endothelial cells |
| HEK-293T | in vitro | DNA | jetPEI |
Cho, C. P. et al. (2013)
PLoS One 8, e62283 Regulation of programmed ribosomal frameshifting by co-translational refolding RNA hairpins | |
| HEK-293, HeLa, MCF7, MEF90 | in vitro | DNA | jetPEI |
Zhao, L. J. et al. (2009)
BMC Mol Biol 10, 89 Interaction of ZEB and histone deacetylase with the PLDLS-binding cleft region of monomeric C-terminal binding protein 2 | |
| HEK-293 | in vitro | DNA | jetPEI |
Condliffe, S. B. et al. (2013)
J Biol Chem 288, 33873-83 The E1015K variant in the synprint region of the CaV2.1 channel alters channel function and is associated with different migraine phenotypes | |
| HEK-293T | in vitro | DNA | jetPEI |
de Chassey, B. et al. (2013)
EMBO Rep 14, 938-44 Structure homology and interaction redundancy for discovering virus-host protein interactions | |
| BG1, HeLa | in vitro | DNA, siRNA | INTERFERin, jetPEI |
Docquier, A. et al. (2013)
Mol Endocrinol 27, 1429-41 Negative regulation of estrogen signaling by ERbeta and RIP140 in ovarian cancer cells | |
| HeLa | in vitro | DNA | jetPEI |
Feng, H. et al. (2013)
Cell Death Dis 4, e870 Calumenin-15 facilitates filopodia formation by promoting TGF-beta superfamily cytokine GDF-15 transcription | |
| HeLa | in vitro | DNA | jetPEI |
Germain, M. A. et al. (2013)
Mol Cell Proteomics , Elucidating Novel Hepatitis C Virus/Host Interactions Using Combined Mass Spectrometry and Functional Genomics Approaches. | |
| C2C12, HCT 116, MCF7 | in vitro | DNA, siRNA | jetPEI |
Guillaume, E. et al. (2013)
J Cell Sci , Flotillin micro-domains stabilize Cadherins at cell-cell junctions | |
| MIN6 | in vitro | DNA | jetPEI |
Hofmeister-Brix, A. et al. (2013)
Biochem J 456, 173-84 The ubiquitin-proteasome system regulates the stability and activity of the glucose sensor glucokinase in pancreatic beta-cells |
