DNA及SiRNA转染试剂jetPRIME

DNA及SiRNA转染试剂jetPRIME
PT-114-15（101000046）
jetPRIME® Versatile DNA/siRNA transfection reagent

jetPRIME

规格：1.5 mL of transfection reagent + 2 x (60 mL of jetPRIME buffer)
PT-114-15（101000046）

Specifications

Reagent	jetPRIME®
Molecule delivered	DNA, siRNA, DNA & siRNA
Applications	Plasmid transfection Genome editing Virus production siRNA transfection Co-delivery of different nucleic acids
Cell types	Adherent cell lines grown in presence of serum
Number of transfections	1.5 ml of jetPRIME® transfection reagent is sufficient to perform up to 1500 transfections in 24-well plates or 375 transfections in 6-well plates
Storage	5°C ± 3°C, stable for at least one year when stored appropriately
Provided with	jetPRIME buffer

Ordering information

“JETPrimE比其他试剂具有更好的转染效率，并注意到细胞死亡少得多。细胞似乎不介意用转染试剂治疗，无论如何都不需要改变培养基。我已经订购了一个更大的JETHEAD卷，已经使用了一半！可能很快会订购更多！“Kimberley S.，英国皇家兽医学院


“我已经订购了。这是非常有效和易于使用！转染后细胞处于良好状态。好产品！“-科西马R，埃朗根大学医院，DE


“非常好的转染效率，更好的价值和易用性”——Sean H.，MD安德森癌症中心，美国


“Jeta”毒性低，DNA量少，可实现转染试剂的可比表达。出于上述原因，我更喜欢使用jetPRIME而不是其他试剂。


G“我们仍然喜欢JETHEAST转染试剂。它确实是好的能满足我们永生化的初级MEF的试剂，并允许我们实验室的两个项目比我们初计划的慢病毒策略进展得更快。好极了！“Jeetayu B.，艾伯特爱因斯坦医学院，美国


“第二天我订购了一些JETHELP试剂的效率让我非常惊喜。众所周知，MCF7细胞很难被转染，而且它们是我们实验室中的主要细胞系，所以我非常感谢以合理的成本使用这种试剂。


“非常感谢我收到的JETHEAD样品，我非常高兴并感谢您出色的服务。我向许多研究人员和学生推荐了这种试剂，他们很快就会订购。

jetPRIME^® is a powerful transfection reagent for day-to-day experiments. It leads to unusually high percentage of transfected adherent cell lines of various origins as well as primary cells.

Superior transfection efficiencies ranging between 70 and 90% were obtained with jetPRIME^® reagent versus the top competitor’s reagent for several commonly used cell lines (Fig.1 & Fig.2).

Fig. 1: Comparative transfection efficiency of jetPRIME versus its main competitor. Transfection efficiency was assessed by FACS analysis in various cell lines 24 h after transfection in 24-well plates. Conditions were used according to the manufacturer’s recommendation for both Lipofectamine^® 2000 and for jetPRIME^®. Fig. 2: Comparative transfection efficiency of jetPRIME^® versus Lipofectamine^® 3000. Transfection efficiency was assessed by FACS analysis in various cell lines 24 h after transfection in 96-well plates or 24-well plates. Conditions were used according to the manufacturer’s recommendation for lipofectamine^® 3000 and for jetPRIME^®.

Cost-effective: less DNA and less reagent

jetPRIME^® is a powerful in vitro transfection reagent that requires a small amount of reagent and plasmid DNA, making its use very cost-effective (Table 1).

Table 1: Recommended conditions to use. Amounts of DNA and reagent (jetPRIME^® and competitors) per well in 6-well plate for transfection according to manufacturers’ recommendations.

In addition to reducing costs, using less DNA also minimizes adverse cytotoxic effects triggered by transfection. Hence, jetPRIME^® is the reagent of choice for high transfection efficiency with excellent cell viability.

文献：

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