oxidized Low Density Lipoprotein, labeled with 1,1’-dioctadecyl – 3,3,3’,3’-tetram et hyl-indocarbocyanine perchlorate, which is highly fluorescent lipophilic dyes that diffuse into the hydrophobic portion of the LDL complex without affecting the LDL-specific binding of the apoprotein. DiI-Ox-LDL is used to label both vascular endothelial cells and macrophages. It can be used to identify and/or isolate these cells from mixed cell populations and investigate uptake and binding of modified LDL by different cell types. DiI has been excited at 488nm or 514nm and the emission is detected at 565 nm.
High Oxidized LDL (High OxLDL)
Our Human LDL is oxidized using Cu2SO4 (oxidant) in PBS. Oxidation is terminated by adding excess EDTA-Na2. Each lot is analyzed on agarose gel electrophoresis for migration versus LDL. This lot of OxLDL migrates 2.0fold further than the native LDL.