GenJet™ In Vitro DNA Tranfection Reagent for PC-3 Cells
库存:
现货 大量
供应商:
济南美中清水湾生物科技有限公司
保存条件:
4°C
规格:
毫升/支
Description
GenJet™ DNA In Vitro Tranfection Reagent for PC-3 is pre-optimized for transfecting PC-3 cells. This human prostate cancer cell line very sensitive to liposome based mediated toxicity, leading high percentage of cell death. With the pre-optimized DNA transfection reagent, up to 60% efficiency can be achieved.
Refer to the following optimal transfection conditions for maximal transfection efficiency on PC-3 cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 50 to 100 transfections in 6 well plates.
Summary of Optimal Transfection Conditions: Confluence on the day of transfection Cell culture conditions GenJet™ (µl) : DNA (µg) Ratio Diluent for DNA and Transfection Reagent Incubation Time to Form GenJet™/DNA Complex Presence of Serum/Antibiotics during Transfection Change Medium After Transfection Maximal Efficiency