- ¥1560
- SignaGen
- 美国
- SL100489-PC12
- 2026年04月24日
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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
一年
- 英文名：
GenJet™ In Vitro DNA Tranfection Reagent for PC-12 Cell
- 库存：
现货 大量
- 供应商：
济南美中清水湾生物科技有限公司
- 保存条件：
4°C
- 规格：
毫升/支
GenJet™ DNA In Vitro Tranfection Reagent for PC12 cell is pre-optimized for most efficiently transfecting PC12 cells. PC12 is a cell line derived from a pheochromocytoma of the rat adrenal medulla. PC12 cells stop dividing and terminally differentiate when treated with nerve growth factor. This makes PC12 cells useful as a model system for neuronal differentiation.
Refer to the following optimal transfection conditions for maximal transfection efficiency on PC12 cells. GenJet™ reagent, 1.0 ml, is sufficient for 660 transfections in 24 well plates or 330 transfections in 6 well plates.
| Summary of Optimal Transfection Conditions: Confluence on the day of transfection Cell culture conditions GenJet™ (µl) : DNA (µg) Ratio Diluent for DNA and Transfection Reagent Incubation Time to Form GenJet™/DNA Complex Presence of Serum/Antibiotics during Transfection Change Medium After Transfection Maximal Efficiency Transfection Results: Reporter Gene Plasmid Efficiency (GFP %) |
~80% DMEM with 4.5 g/L glucose, 10% FBS 3:1 Serum-free DMEM with 4.5 g/L glucose 15 minutes at RT Yes Yes, ~5 Hours After Transfection 48 hours EGFP pEGFP-N3 (CMV promoter) 50% |
Store at 4 °C. If stored properly, the product is stable for 12 months or longer
Data Sheet
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文献和实验in a variant PC12 cell line (N1). Here, the functional role of JNK in N1 cells is further investigated. We show that NGF treatment, which induces extensive neurite branching and cell soma enlargement in the N1 cells, stimulates a biphasic activation
细胞分化神经纤维：要求：（1）细胞密度: 2.5-10×104 cell/cm2（2）塑料培养皿的包被。上述细胞培养24小时后，换入含2.5S NGF(50ng/ml)的DMEM完全培养液，以后隔天换液（含NGF）；取NGF分化5-10天后的PC12细胞用于实验。（一般情况下，NGF诱导10-14天时，NGF可使PC12细胞长出神经纤维，并形成致密的网络丁香园liandchu的问题：我现在正在做一种神经营养因子的功能,它能促进神经突起的生长,我用的细胞模型是pc12细胞,但pc12 不容易贴壁
observation. This reduces autofluorescence. 4. Store cells by suspending in 90% medium, 10% DMSO, freezing slowly as for other cell lines before transfering to liquid nitrogen. Reference L.A.Greene, J.M.Aletta, A.Rukenstein and S.H.Green (1987) PC12
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