- ¥1560
- SignaGen
- 美国
- SL100489-MB231
- 2026年01月13日
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- 询价记录
- 文献和实验
- 技术资料
- 保质期：
一年
- 英文名：
GenJet™ In Vitro DNA Tranfection Reagent for MDA-MB231 Cells
- 库存：
现货 大量
- 供应商：
济南美中清水湾生物科技有限公司
- 保存条件：
4°C
GenJet™ DNA In Vitro Tranfection Reagent for MB231 is pre-optimized for transfecting MDA-MB231 cells. The MDA-MB-231 breast cancer cell line was obtained from a pleural effusion of a 51-year-old white female in October 17, 1973, by Dr. R. Calleau at M. D. Anderson Cancer Center. The patient underwent a mastectomy and radiotherapy prior to the removal of the breast cancer specimen. After 52 passages, the karyotype displayed chromosome numbers ranging between 52 and 62, where the modal chromosome number was 57. With epithelial-like morphology, the MDA-MB-231 breast cancer cells appear phenotypically as spindle shaped cells. In vitro, the MDA-MB-231 cell line has an invasive phenotype. It has abundant activity in both the Boyden chamber chemoinvasion and chemotaxis assay. The MDA-MB-231 cell line is also able to grow on agarose, an indicator of transformation and tumorigenicity, and displays a relatively high colony forming efficiency.
The "Shaved Cell Transfection" protocol was optimized to achieve maximal transfection efficiency. Refer to the following optimal transfection conditions for maximal transfection efficiency on MB231 cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 transfections in 24 well plates or 150 transfections in 6 well plates.
| Summary of Optimal Shaved CellTransfection Conditions: Cell culture medium Confluency of cell on the day of shaving Cell density for plating GenJet™ (µl) : DNA (µg) Ratio Diluent for DNA and Transfection Reagent Incubation Time to Form GenJet™/DNA Complex Incubation time of shaved cells with transfection complex Maximal Efficiency Transfection Results: Reporter Gene Plasmid Efficiency (GFP %) |
DMEM with 4.5 g/L glucose, 10% FBS 95~100% 1.3x10^5 cells per square centimeter 4:1 Serum-free DMEM with 4.5 g/L glucose 12 minutes at RT 20 minutes at 37 degree 48 hours EGFP pEGFP-N3 (CMV promoter) 70% |
Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer
A Picture Showing Transfection Efficiency of GenJet™ Reagent on MDA-MB231 Cells. MDA-MB231 cells were grown per ATCC recommended culture medium and transfected with pEGFP-N3 by GenJet™. The efficiency was checked 24 hours post transfection
Data Sheet
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文献和实验相关专题 总有一种转染方法适合你 近日，罗氏公司新推出了两款高性能的DNA转染试剂，为其细胞分析产品线再添新军。这两款名为X-tremeGENE 9和X-tremeGENE HP的转染试剂沿用了X-tremeGENE siRNA 转染试剂的前缀，应该与大名鼎鼎的Fugene 6不属于一类。据罗氏介绍，这两个产品是多组分的独特试剂，并非脂质体。它们能够提供高的转染效率、重复性和细胞存活率。 这两款新产品都融合了罗氏二十
DNA Delivery to Cells in Culture Using Peptides
), serum has minimal effect on gene expression with the H-K triplex. Poly-L -lysine (K) or H-K polymers were first incubated with the PCI-Luc for 30 min followed by incubating with DOTAP liposomes. After incubating the triplex with MDA-MB-435 cells for 4 h
Activation and Differentiation of Mesenchymal Stem Cells
finding, hMSCs become activated and resemble carcinoma-associated myofibroblasts upon prolonged exposure to conditioned medium from MDAMB231 human breast cancer cells. Here, we show that keratinocyte-conditioned medium (KCM) induces differentiation of MSCs
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