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- 详细信息
- 文献和实验
- 技术资料
- 保质期:
一年
- 英文名:
GenJet™ In Vitro DNA Tranfection Reagent for C2C12 Cells
- 库存:
现货 大量
- 供应商:
济南美中清水湾生物科技有限公司
- 保存条件:
4°C
- 规格:
毫升/支
Description
GenJet™ DNA In Vitro Tranfection Reagent for C2C12 Cell is pre-optimized for transfecting C2C12 cells. C2C12 cells were originally obtained by Yaffe and Saxel (1977) through selective serial passage of myoblasts cultured from the thigh muscle of C3H mice 70 h after a crush injury. These cells were shown to be capable of differentiation. C2C12 cells are a useful model to study the differentiation of non-muscle cells to skeletal muscle cells (e.g myosin phosphorylation mechanisms) and express muscle proteins and the androhen receptor (AR).
Refer to the following optimal transfection conditions for maximal transfection efficiency on C2C12 cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 150 to 300 transfections in 6 well plates.
| Summary of Optimal Shaved CellTransfection Conditions: |
|
Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer
Data Sheet ![]()
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文献和实验相关专题 总有一种转染方法适合你 近日,罗氏公司新推出了两款高性能的DNA转染试剂,为其细胞分析产品线再添新军。这两款名为X-tremeGENE 9和X-tremeGENE HP的转染试剂沿用了X-tremeGENE siRNA 转染试剂的前缀,应该与大名鼎鼎的Fugene 6不属于一类。据罗氏介绍,这两个产品是多组分的独特试剂,并非脂质体。它们能够提供高的转染效率、重复性和细胞存活率。 这两款新产品都融合了罗氏二十
在实验过程中我们可能会碰到各种各样的细胞,不同的细胞培养和感染可能会有所不同,像很多细胞系的培养和感染可能会比较容易,但也有细胞比较「娇嫩」,普通的 DNA 质粒根本就转不进去,即使是病毒载体感染,也有不少难题:有些细胞的慢病毒感染能力偏弱,需要多次重复感染;有些细胞本身十分敏感且脆弱,慢病毒感染对细胞的状态影响非常大;有些细胞是悬浮细胞,进一步增大了病毒感染的难度。那么针对不同的细胞我们都有哪些培养和感染注意事项呢? 一、贴壁细胞 贴壁细胞(adherent cells),这类细胞的生长必须
Evaluation of Cytocompatibility and Cell Response to Boron Nitride Nanotubes
reports on preliminary cytocompatibility studies carried out on human neuroblastoma (SH-SY5Y) and on mouse myoblast (C2C12) cell lines as model of neural and skeletal muscle cells, respectively, highlighting the methods that allowed us to evaluate the main
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