- ¥1560
- SignaGen
- 美国
- Catalog #: SL100489-CHO
- 2026年01月07日
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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
一年
- 英文名：
GenJet™ In Vitro DNA Tranfection Reagent for CHO Cells
- 库存：
现货 大量
- 供应商：
济南美中清水湾生物科技有限公司
- 保存条件：
4°C
GenJet™ DNA In Vitro Tranfection Reagent for CHO is pre-optimized for transfecting Chinese Hamster Ovary (CHO) cells. Refer to the following optimal transfection conditions for maximal transfection efficiency on CHO cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 50 to 100 transfections in 6 well plates.
| Summary of Optimal Transfection Conditions: Confluence on the day of transfection Cell culture conditions GenJet™ (µl) : DNA (µg) Ratio Diluent for DNA and Transfection Reagent Incubation Time to Form GenJet™/DNA Complex Presence of Serum/Antibiotics during Transfection Change Medium After Transfection Maximal Efficiency Transfection Results: Reporter Gene Plasmid Efficiency (GFP %) |
~90% DMEM with 4.5 g/L glucose, 10% FBS 3:1 Serum-free DMEM with 4.5 g/L glucose 15 minutes at RT Yes Yes, 16~24 Hours After Transfection 48 hours EGFP pEGFP-N3 (CMV promoter) 90% |
Store at 4 °C. If stored properly, the product is stable for 12 months or longer
A Picture Showing Transfection Efficiency of GenJet™ Reagent on CHO Cells. CHO cells were grown per ATCC recommended culture medium and transfected with pEGFP-N3 by GenJet™. The efficiency was checked 24 hours post transfection
Data Sheet
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文献和实验Establishment of Stable Transfectant of CHO Lec Cells
Purpose and Backgrounds CHO lec 3.2.8.1 cells CHO Lec 3.2.8.1 cells have four independent mutations in the N- and O- glycosylation pathways (Stanley, 1989). N-linked carbohydrates produced by CHO Lec 3.2.8.1 cells are all of the high
Harvesting GPI-Anchored Proteins From CHO Cells
Glycosyl-phosphatidylinositol (GPI)-anchored proteins bound to the outer surface of cell membranes (1 ,2 ) can be selectively released in a soluble form by the action of a highly specific bacterial enzyme, phosphatidylinositol-phospholipase
Stable Expression of Chimeric Heavy Chain Antibodies in CHO Cells
Camelid single domain antibodies fused to noncamelid Fc regions, also called chimeric heavy chain antibodies (cHCAb), offer great potential as therapeutic and diagnostic candidates due to their relatively small size (80 kDa) and intact Fc
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