GenJet™ In Vitro DNA Tranfection Reagent for CHO Cells
库存:
现货 大量
供应商:
济南美中清水湾生物科技有限公司
保存条件:
4°C
Description
GenJet™ DNA In Vitro Tranfection Reagent for CHO is pre-optimized for transfecting Chinese Hamster Ovary (CHO) cells. Refer to the following optimal transfection conditions for maximal transfection efficiency on CHO cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 50 to 100 transfections in 6 well plates.
Summary of Optimal Transfection Conditions: Confluence on the day of transfection Cell culture conditions GenJet™ (µl) : DNA (µg) Ratio Diluent for DNA and Transfection Reagent Incubation Time to Form GenJet™/DNA Complex Presence of Serum/Antibiotics during Transfection Change Medium After Transfection Maximal Efficiency
~90% DMEM with 4.5 g/L glucose, 10% FBS 3:1 Serum-free DMEM with 4.5 g/L glucose 15 minutes at RT Yes Yes, 16~24 Hours After Transfection 48 hours
EGFP pEGFP-N3 (CMV promoter) 90%
Storage Condition Store at 4 °C. If stored properly, the product is stable for 12 months or longer
A Picture Showing Transfection Efficiency of GenJet™ Reagent on CHO Cells. CHO cells were grown per ATCC recommended culture medium and transfected with pEGFP-N3 by GenJet™. The efficiency was checked 24 hours post transfection