AssayPro—Human vWF ELISA Kit（血

AssayMax Human vWF ELISA Kit
血管性血友病因子vWF酶联免疫试剂盒

Introduction
Von Willebrand factor (VWF) is a multimeric glycoprotein that circulates in blood forming a noncovalent complex with procoagulant factor VIII (1). During normal homeostasis, the larger multimers of VWF are responsible for facilitating platelet plug formation by forming a bridge between platelet glycoprotein IB and exposed collagen in the subendothelium (2 - 3). The congenital dysfunctional state of VWF causes a moderate to severe bleeding diathesis-von Willebrand disease (VWD).

Principle of the Assay
The AssayMax VWF ELISA kit is designed for detection of human VWF in plasma, serum, and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures VWF in less than 5 hours. A monoclonal antibody specific for VWF has been pre-coated onto a microplate. Human VWF in standards and samples is sandwiched by the immobilized antibody and the biotinylated antibody specific for VWF, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.