AssayPro—Human Factor VII ELIS

Introduction
Factor VII (FVII) is a vitamin K-dependent plasma glycoprotein that is synthesized in the liver and circulates in blood as a single-chain inactive zymogen with a molecular mass of 50 kDa (1). Upon tissue damage and vascular injury, the cell surface receptor and cofactor tissue factor binds and allosterically activates FVII to its active form, FVIIa. The tissue factor/FVIIa complex catalyzes the conversion of both factor IX to factor IXa and factor X to factor Xa to initiate coagulation via the extrinsic pathway (2 - 3). Very low levels of FVII are associated with severe coagulation disorders (4). Elevated plasma levels of FVII coagulant activity constitute an independent risk factor for fatal outcomes of coronary heart disease in middle-aged men (5).

Principle of the Assay
The AssayMax Human Factor VII (FVII) ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of human factor VII and factor VIIa in plasma, serum, and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures total FVII in less than 4 hours. A monoclonal antibody specific for FVII has been pre-coated onto a 96-well microplate with removable strips. FVII in standards and samples is sandwiched by the immobilized antibody and the biotinylated antibody specific for FVII, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.