Human von Willebrand Factor cleaving protease,ADAMTS-13/vWF-cp ELISA Kit
精密度:
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Human von Willebrand Factor cleaving protease,ADAMTS-13/vWF-cp ELISA Kit
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human ADAMTS-13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample Serum(n=4)
1:1000 Average % 88
Range % 80-92
1:2000 Average % 98
Range % 91-105
1:4000 Average % 101
Range % 92-110
1:8000 Average % 94
Range % 86-98
Human von Willebrand Factor cleaving protease,ADAMTS-13/vWF-cp ELISA Kit
回收率:
The recovery of human ADAMTS-13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 94 89-98
EDTA plasma (n=4) 96 90-100