AssayPro—Mouse TAT Complex ELI

Introduction
Thrombin-antithrombin (TAT) complexes formed following the neutralization of thrombin by antithrombin III (ATIII) have been used as a surrogate marker for thrombin generation (1). High plasma levels of TAT complexes have been suggested to alter hemostatic activation in argentine hemorrhagic fever (2), chronic dialysis patients (3), and toxemia of pregnancy (4). Whereas low plasma levels of TAT complexes are found in type 1 (insulin-dependent) diabetes (5), neonatal respiratory distress syndrome (6), and primary untreated cancer (7). TAT complexes are a useful marker to predict morphological changes in chronic aortic dissection (8).
凝血酶-抗凝血酶复合物（TAT）是凝血酶被抗凝血酶III中和后形成的，是用于证实凝血酶生成的一个重要标志物（1）、高水平的TAT复合物改变了止血剂的活化作用，在出血性发烧疾病（2）、慢性透析患者（3）、以及孕妇的毒血症（4）。然而，低水平的TAT复合物与I型糖尿病（5）、新生儿呼吸窘迫综合症（6）、及原发性肿瘤有关（7）。TAT是血栓前状态十分重要的检测指标（8）。


Principle of the Assay
The AssayMax Mouse TAT Complexes ELISA kit is designed for detection of mouse TAT complexes in plasma and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures TAT complexes in less than 5 hours. A polyclonal antibody specific for mouse thrombin has been pre-coated onto a microplate. TAT complexes in standards and samples are sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for mouse antithrombin, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.