pBiFC-HA-NELFCD-VC155 plasmid

pBiFC-HA-NELFCD-VC155 plasmid

PVT86266

pBiFC-HA-NELFCD-VC155 plasmid Information

Plasmid name: pBiFC-HA-NELFCD(human)-VC155
Alias: NM_198976.4;HSPC130; NELF-C; NELF-D; TH1; TH1L;
Gene lenght: 1743bp
Host: Mammalian cells
Use(s): Bimolecular fluorescence
Fragment type: BIFC
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone: pBiFC-VC155
Forward primer: SV40 intron-F1
Reverse primer:
Copy number: High
Induction:

pBiFC-HA-NELFCD-VC155 plasmid Description

pBiFC-HA-NELFCD-VC155 Whether they can interact. The N-terminal of the NELFCD gene is fused to express an HA tag, facilitating experiments such as protein immunoprecipitation and co-immunoprecipitation.

pBiFC-HA-NELFCD-VC155 plasmid Sequence

1 ATGTACCCAT ACGATGTTCC AGATTACGCT CTTATGGCCA TGGAGGCCCG AATTCGGATG 61 GACGAGGACT ACTACGGGAG CGCGGCCGAG TGGGGCGACG AGGCTGACGG CGGCCAGCAG 121 GAGGATGATT CTGGAGAAGG AGAGGATGAT GCGGAGGTTC AGCAAGAATG CCTGCATAAA 181 TTTTCCACCC GGGATTATAT CATGGAACCC TCCATCTTCA ACACTCTGAA GAGGTATTTT 241 CAGGCAGGAG GGTCTCCAGA GAATGTTATC CAGCTCTTAT CTGAAAACTA CACCGCTGTG 301 GCCCAGACTG TGAACCTGCT GGCCGAGTGG CTCATTCAGA CAGGTGTTGA GCCAGTGCAG 361 GTTCAGGAAA CTGTGGAAAA TCACTTGAAG AGTTTGCTGA TCAAACATTT TGACCCCCGC 421 AAAGCAGATT CTATTTTTAC TGAAGAAGGA GAGACCCCAG CGTGGCTGGA ACAGATGATT 481 GCACATACCA CGTGGCGGGA CCTTTTTTAT AAACTGGCTG AAGCCCATCC AGACTGTTTG 541 ATGCTGAACT TCACCGTTAA GCTTATTTCT GACGCAGGGT ACCAGGGGGA GATCACCAGT 601 GTGTCCACAG CATGCCAGCA GCTAGAAGTG TTCTCGAGAG TGCTCCGGAC CTCTCTAGCT 661 ACAATTTTAG ATGGAGGAGA AGAAAACCTT GAAAAAAATC TCCCTGAGTT TGCCAAGATG 721 GTGTGCCACG GGGAGCACAC GTACCTGTTT GCCCAGGCCA TGATGTCCGT GCTGGCCCAG 781 GAGGAGCAGG GGGGCTCCGC TGTGCGCAGG ATCGCCCAGG AAGTGCAGCG CTTTGCCCAG 841 GAGAAAGGTC ATGACGCCAG TCAGATCACA CTAGCCTTGG GCACAGCTGC CTCCTACCCC 901 AGGGCCTGCC AGGCTCTCGG GGCCATGCTG TCCAAAGGAG CCCTGAACCC TGCTGACATC 961 ACCGTCCTGT TCAAGATGTT CACAAGCATG GACCCTCCTC CGGTTGAACT TATCCGCGTT 1021 CCAGCCTTCC TGGACCTGTT CATGCAGTCA CTCTTTAAAC CAGGGGCTCG GATCAACCAG 1081 GACCACAAGC ACAAATACAT CCACATCTTG GCGTACGCAG CAAGCGTGGT TGAGACCTGG 1141 AAGAAGAACA AGCGAGTGAG CATCAATAAA GATGAGCTGA AGTCAACGTC AAAAGCTGTC 1201 GAAACCGTTC ACAATTTGTG TTGCAACGAG AACAAAGGGG CCTCTGAACT AGTGGCAGAA 1261 TTGAGCACAC TTTATCAGTG TATTAGGTTT CCAGTGGTAG CAATGGGTGT GCTGAAGTGG 1321 GTGGATTGGA CTGTATCAGA ACCAAGGTAC TTTCAGCTGC AGACTGACCA TACCCCTGTC 1381 CACCTGGCGT TGCTGGATGA GATCAGCACC TGCCACCAGC TCCTGCACCC CCAGGTCCTG 1441 CAGCTGCTTG TTAAGCTTTT TGAGACTGAG CACTCCCAGC TGGACGTGAT GGAGCAGCTT 1501 GAGTTGAAGA AGACACTGCT GGACAGGATG GTTCACCTGC TGAGTCGAGG TTATGTACTT 1561 CCTGTTGTCA GTTACATCCG AAAGTGTCTG GAGAAGCTGG ACACTGACAT TTCACTCATT 1621 CGCTATTTTG TCACTGAGGT GCTGGACGTC ATTGCTCCTC CTTATACCTC TGACTTCGTG 1681 CAACTTTTCC TCCCCATCCT GGAGAATGAC AGCATCGCAG GTACCATCAA AACGGAAGGC 1741 GAGCATGACC CTGTGACGGA GTTTATAGCT CACTGCAAAT CTAACTTCAT CATGGTGAAC 1801 GGTACCCGTC CGGCGTGCAA AATCCCGAAC GACCTGAAAC AGAAAGTCAT GAACCACGAC 1861 AAGCAGAAGA ACGGCATCAA GGCCAACTTC AAGATCCGCC ACAACATCGA GGACGGCGGC 1921 GTGCAGCTCG CCGACCACTA CCAGCAGAAC ACCCCCATCG GCGACGGCCC CGTGCTGCTG 1981 CCCGACAACC ACTACCTGAG CTACCAGTCC AAACTGAGCA AAGACCCCAA CGAGAAGCGC 2041 GATCACATGG TCCTGCTGGA GTTCGTGACC GCCGCCGGGA TCACTCTCGG CATGGACGAG 2101 CTGTACAAGT AAGCGGCCGC GGGGATCCAG ACATGATAAG ATACATTGAT GAGTTTGGAC 2161 AAACCACAAC TAGAATGCAG TGAAAAAAAT GCTTTATTTG TGAAATTTGT GATGCTATTG 2221 CTTTATTTGT AACCATTATA AGCTGCAATA AACAAGTTAA CAACAACAAT TGCATTCATT 2281 TTATGTTTCA GGTTCAGGGG GAGGTGTGGG AGGTTTTTTC GGATCCTCTA GAGTCGATCT 2341 GCAGGCATGC TAGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC 2401 CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT 2461 AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA 2521 ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA 2581 TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC 2641 GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG 2701 CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT 2761 TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA 2821 GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT 2881 CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC 2941 CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG 3001 TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT 3061 TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG 3121 CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA 3181 AGTGGTGGCC TAACTACGGC TACACTAGAA GGACAGTATT TGGTATCTGC GCTCTGCTGA 3241 AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG 3301 GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG 3361 AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG 3421 GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT 3481 GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT 3541 TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC 3601 TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA 3661 TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG 3721 GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT 3781 GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA 3841 TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT 3901 CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT 3961 TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG 4021 CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG 4081 AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG 4141 CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA 4201 AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT 4261 AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT 4321 GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT 4381 GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA 4441 TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT 4501 TTCCCCGAAA AGTGCCACCT GACGTCTAAG AAACCATTAT TATCATGACA TTAACCTATA 4561 AAAATAGGCG TATCACGAGG CCCTTTCGTC TCGCGCGTTT CGGTGATGAC GGTGAAAACC 4621 TCTGACACAT GCAGCTCCCG GAGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA 4681 GACAAGCCCG TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG 4741 CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATATGCG GTGTGAAATA CCGCACAGAT 4801 GCGTAAGGAG AAAATACCGC ATCAGGCGCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG 4861 AAGGGCGATC GGTGCGGGCC TCTTCGCTAT TACGCCAGCT GGCGAAAGGG GGATGTGCTG 4921 CAAGGCGATT AAGTTGGGTA ACGCCAGGGT TTTCCCAGTC ACGACGTTGT AAAACGACGG 4981 CCAGTGAGTT CGAGCTTGCA TGCCTGCAGG TCGTTACATA ACTTACGGTA AATGGCCCGC 5041 CTGGCTGACC GCCCAACGAC CCCCGCCCAT TGACGTCAAT AATGACGTAT GTTCCCATAG 5101 TAACGCCAAT AGGGACTTTC CATTGACGTC AATGGGTGGA GTATTTACGG TAAACTGCCC 5161 ACTTGGCAGT ACATCAAGTG TATCATATGC CAAGTACGCC CCCTATTGAC GTCAATGACG 5221 GTAAATGGCC CGCCTGGCAT TATGCCCAGT ACATGACCTT ATGGGACTTT CCTACTTGGC 5281 AGTACATCTA CGTATTAGTC ATCGCTATTA CCATGGTGAT GCGGTTTTGG CAGTACATCA 5341 ATGGGCGTGG ATAGCGGTTT GACTCACGGG GATTTCCAAG TCTCCACCCC ATTGACGTCA 5401 ATGGGAGTTT GTTTTGGCAC CAAAATCAAC GGGACTTTCC AAAATGTCGT AACAACTCCG 5461 CCCCATTGAC GCAAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA AGCAGAGCTC 5521 GTTTAGTGAA CCGTCAGATC GCCTGGAGAC GCCATCCACG CTGTTTTGAC CTCCATAGAA 5581 GACACCGGGA CCGATCCAGC CTCCGGACTC TAGAGGATCC GGTACTAGAG GAACTGAAAA 5641 ACCAGAAAGT TAACTGGTAA GTTTAGTCTT TTTGTCTTTT ATTTCAGGTC CCGGATCCGG 5701 TGGTGGTGCA AATCAAAGAA CTGCTCCTCA GTGGATGTTG CCTTTACTTC TAGGCCTGTA 5761 CGGAAGTGTT ACTTCTGCTC TAAAAGCTGC GGAATTGTAC CCGCGGGCCC ACC

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.