pHAGE-Usp13-Flag-Puro plasmid

pHAGE-Usp13-Flag-Puro plasmid

PVT86732

pHAGE-Usp13-Flag-Puro plasmid Information

Plasmid name: pHAGE-Usp13(mouse)-Flag-Puro
Alias: NM_001013024.2;2700071E21Rik; IsoT-3; ISOT3
Gene lenght: 2574bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone: pHAGE_puro
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pHAGE-Usp13-Flag-Puro plasmid Description

pHAGE-Usp13-Flag-Puro This plasmid is suitable for gene overexpression in mammalian cells. The CMV strong promoter carried by the plasmid can initiate the transcription of downstream genes, promoting the C-terminal fusion expression of the FLAG tag, which facilitates the detection, localization, and purification of the expressed fusion protein using FLAG antibodies. Additionally, the plasmid contains HIV-1 Ψ and 3'/5' LTR elements for lentivirus packaging, facilitating the stable integration of the gene into host cells. The plasmid carries a Puro resistance gene for screening host cells expressing the target gene.

pHAGE-Usp13-Flag-Puro plasmid Sequence

1 ACCTTCTACT CCTCCCCTAG TCAGGAAGTT CCCCCCCGCC CCGCAGCTCG CGTCGTGCAG 61 GACGTGACAA ATGGAAGTAG CACGTCTCAC TAGTCTCGTG CAGATGGACA GCACCGCTGA 121 GCAATGGAAG CGGGTAGGCC TTTGGGGCAG CGGCCAATAG CAGCTTTGCT CCTTCGCTTT 181 CTGGGCTCAG AGGCTGGGAA GGGGTGGGTC CGGGGGCGGG CTCAGGGGCG GGCTCAGGGG 241 CGGGGCGGGC GCCCGAAGGT CCTCCGGAGG CCCGGCATTC TGCACGCTTC AAAAGCGCAC 301 GTCTGCCGCG CTGTTCTCCT CTTCCTCATC TCCGGGCCTT TCGACCTGCA GCCCAAGCTT 361 ACCATGACCG AGTACAAGCC CACGGTGCGC CTCGCCACCC GCGACGACGT CCCCAGGGCC 421 GTACGCACCC TCGCCGCCGC GTTCGCCGAC TACCCCGCCA CGCGCCACAC CGTCGATCCG 481 GACCGCCACA TCGAGCGGGT CACCGAGCTG CAAGAACTCT TCCTCACGCG CGTCGGGCTC 541 GACATCGGCA AGGTGTGGGT CGCGGACGAC GGCGCCGCGG TGGCGGTCTG GACCACGCCG 601 GAGAGCGTCG AAGCGGGGGC GGTGTTCGCC GAGATCGGCC CGCGCATGGC CGAGTTGAGC 661 GGTTCCCGGC TGGCCGCGCA GCAACAGATG GAAGGCCTCC TGGCGCCGCA CCGGCCCAAG 721 GAGCCCGCGT GGTTCCTGGC CACCGTCGGC GTCTCGCCCG ACCACCAGGG CAAGGGTCTG 781 GGCAGCGCCG TCGTGCTCCC CGGAGTGGAG GCGGCCGAGC GCGCCGGGGT GCCCGCCTTC 841 CTGGAGACCT CCGCGCCCCG CAACCTCCCC TTCTACGAGC GGCTCGGCTT CACCGTCACC 901 GCCGACGTCG AGGTGCCCGA AGGACCGCGC ACCTGGTGCA TGACCCGCAA GCCCGGTGCC 961 TGAATCGATA GATCCTAATC AACCTCTGGA TTACAAAATT TGTGAAAGAT TGACTGGTAT 1021 TCTTAACTAT GTTGCTCCTT TTACGCTATG TGGATACGCT GCTTTAATGC CTTTGTATCA 1081 TGCTATTGCT TCCCGTATGG CTTTCATTTT CTCCTCCTTG TATAAATCCT GGTTGCTGTC 1141 TCTTTATGAG GAGTTGTGGC CCGTTGTCAG GCAACGTGGC GTGGTGTGCA CTGTGTTTGC 1201 TGACGCAACC CCCACTGGTT GGGGCATTGC CACCACCTGT CAGCTCCTTT CCGGGACTTT 1261 CGCTTTCCCC CTCCCTATTG CCACGGCGGA ACTCATCGCC GCCTGCCTTG CCCGCTGCTG 1321 GACAGGGGCT CGGCTGTTGG GCACTGACAA TTCCGTGGTG TTGTCGGGGA AATCATCGTC 1381 CTTTCCTTGG CTGCTCGCCT GTGTTGCCAC CTGGATTCTG CGCGGGACGT CCTTCTGCTA 1441 CGTCCCTTCG GCCCTCAATC CAGCGGACCT TCCTTCCCGC GGCCTGCTGC CGGCTCTGCG 1501 GCCTCTTCCG CGTCTTCGCC TTCGCCCTCA GACGAGTCGG ATCTCCCTTT GGGCCGCCTC 1561 CCCGCCTGAG ATCCTTTAAG ACCAATGACT TACAAGGCAG CTGTAGATCT TAGCCACTTT 1621 TTAAAAGAAA AGGGGGGACT GGAAGGGCTA ATTCACTCCC AACGAAGACA AGATCTGCTT 1681 TTTGCTTGTA CTGGGTCTCT CTGGTTAGAC CAGATCTGAG CCTGGGAGCT CTCTGGCTAA 1741 CTAGGGAACC CACTGCTTAA GCCTCAATAA AGCTTGCCTT GAGTGCTTCA AGTAGTGTGT 1801 GCCCGTCTGT TGTGTGACTC TGGTAACTAG AGATCCCTCA GACCCTTTTA GTCAGTGTGG 1861 AAAATCTCTA GCAGTAGTAG TTCATGTCAT CTTATTATTC AGTATTTATA ACTTGCAAAG 1921 AAATGAATAT CAGAGAGTGA GAGGCCCGGG TTAATTAAGG AAAGGGCTAG ATCATTCTTG 1981 AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT 2041 TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT 2101 TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA 2161 ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT 2221 TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA 2281 TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA 2341 GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT 2401 GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT 2461 ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA 2521 TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC 2581 CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT 2641 GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA 2701 CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA AACTATTAAC 2761 TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA 2821 AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC 2881 TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC 2941 CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG 3001 ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA 3061 CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA 3121 GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC 3181 GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT 3241 CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA 3301 GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT 3361 TCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA 3421 CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC 3481 CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG 3541 TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG 3601 TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG 3661 CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT 3721 TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC 3781 AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT 3841 TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG 3901 TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA 3961 GTCAGTGAGC GAGGAAGCGG AAGAGCGCCC AATACGCAAA CCGCCTCTCC CCGCGCGTTG 4021 GCCGATTCAT TAATGCAGCA AGCTCATGGC TGACTAATTT TTTTTATTTA TGCAGAGGCC 4081 GAGGCCGCCT CGGCCTCTGA GCTATTCCAG AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA 4141 GGCTTTTGCA AAAAGCTCCC CGTGGCACGA CAGGTTTCCC GACTGGAAAG CGGGCAGTGA 4201 GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA CCCCAGGCTT TACACTTTAT 4261 GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA CAATTTCACA CAGGAAACAG 4321 CTATGACATG ATTACGAATT TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG 4381 TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGGATC AACTGGATAA CTCAAGCTAA 4441 CCAAAATCAT CCCAAACTTC CCACCCCATA CCCTATTACC ACTGCCAATT ACCTGTGGTT 4501 TCATTTACTC TAAACCTGTG ATTCCTCTGA ATTATTTTCA TTTTAAAGAA ATTGTATTTG 4561 TTAAATATGT ACTACAAACT TAGTAGTTGG AAGGGCTAAT TCACTCCCAA AGAAGACAAG 4621 ATATCCTTGA TCTGTGGATC TACCACACAC AAGGCTACTT CCCTGATTAG CAGAACTACA 4681 CACCAGGGCC AGGGGTCAGA TATCCACTGA CCTTTGGATG GTGCTACAAG CTAGTACCAG 4741 TTGAGCCAGA TAAGGTAGAA GAGGCCAATA AAGGAGAGAA CACCAGCTTG TTACACCCTG 4801 TGAGCCTGCA TGGGATGGAT GACCCGGAGA GAGAAGTGTT AGAGTGGAGG TTTGACAGCC 4861 GCCTAGCATT TCATCACGTG GCCCGAGAGC TGCATCCGGA GTACTTCAAG AACTGCTGAT 4921 ATCGAGCTTG CTACAAGGGA CTTTCCGCTG GGGACTTTCC AGGGAGGCGT GGCCTGGGCG 4981 GGACTGGGGA GTGGCGAGCC CTCAGATCCT GCATATAAGC AGCTGCTTTT TGCCTGTACT 5041 GGGTCTCTCT GGTTAGACCA GATCTGAGCC TGGGAGCTCT CTGGCTAACT AGGGAACCCA 5101 CTGCTTAAGC CTCAATAAAG CTTGCCTTGA GTGCTTCAAG TAGTGTGTGC CCGTCTGTTG 5161 TGTGACTCTG GTAACTAGAG ATCCCTCAGA CCCTTTTAGT CAGTGTGGAA AATCTCTAGC 5221 AGTGGCGCCC GAACAGGGAC TTGAAAGCGA AAGGGAAACC AGAGGAGCTC TCTCGACGCA 5281 GGACTCGGCT TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC 5341 CAAAAATTTT GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA 5401 AGCGGGGGAG AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA 5461 AATATAAATT AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC 5521 CTGGCCTGTT AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC 5581 TTCAGACAGG ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG 5641 TGCATCAAAG GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC 5701 AAAACAAAAG TAAGACCACC GCACAGCAAG CGGCCGGCCG CTGATCTTCA GACCTGGAGG 5761 AGGAGATATG AGGGACAATT GGAGAAGTGA ATTATATAAA TATAAAGTAG TAAAAATTGA 5821 ACCATTAGGA GTAGCACCCA CCAAGGCAAA GAGAAGAGTG GTGCAGAGAG AAAAAAGAGC 5881 AGTGGGAATA GGAGCTTTGT TCCTTGGGTT CTTGGGAGCA GCAGGAAGCA CTATGGGCGC 5941 AGCGTCAATG ACGCTGACGG TACAGGCCAG ACAATTATTG TCTGGTATAG TGCAGCAGCA 6001 GAACAATTTG CTGAGGGCTA TTGAGGCGCA ACAGCATCTG TTGCAACTCA CAGTCTGGGG 6061 CATCAAGCAG CTCCAGGCAA GAATCCTGGC TGTGGAAAGA TACCTAAAGG ATCAACAGCT 6121 CCTGGGGATT TGGGGTTGCT CTGGAAAACT CATTTGCACC ACTGCTGTGC CTTGGAATGC 6181 TAGTTGGAGT AATAAATCTC TGGAACAGAT TTGGAATCAC ACGACCTGGA TGGAGTGGGA 6241 CAGAGAAATT AACAATTACA CAAGCTTAAT ACACTCCTTA ATTGAAGAAT CGCAAAACCA 6301 GCAAGAAAAG AATGAACAAG AATTATTGGA ATTAGATAAA TGGGCAAGTT TGTGGAATTG 6361 GTTTAACATA ACAAATTGGC TGTGGTATAT AAAATTATTC ATAATGATAG TAGGAGGCTT 6421 GGTAGGTTTA AGAATAGTTT TTGCTGTACT TTCTATAGTG AATAGAGTTA GGCAGGGATA 6481 TTCACCATTA TCGTTTCAGA CCCACCTCCC AACCCCGAGG GGACCCGACA GGCCCGAAGG 6541 AATAGAAGAA GAAGGTGGAG AGAGAGACAG AGACAGATCC ATTCGATTAG TGAACGGATC 6601 TCGACGGTAT CGCCGAATTC ACAAATGGCA GTATTCATCC ACAATTTTAA AAGAAAAGGG 6661 GGGATTGGGG GGTACAGTGC AGGGGAAAGA ATAGTAGACA TAATAGCAAC AGACATACAA 6721 ACTAAAGAAT TACAAAAACA AATTACAAAA ATTCAAAATT TTCGGGTTTA TTACAGGGAC 6781 AGCAGAGATC CAGTTTGGAC TAGTGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC 6841 GCCTGGCTGA CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT 6901 AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC GGTAAACTGC 6961 CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTACG CCCCCTATTG ACGTCAATGA 7021 CGGTAAATGG CCCGCCTGGC ATTATGCCCA GTACATGACC TTATGGGACT TTCCTACTTG 7081 GCAGTACATC TACGTATTAG TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAT 7141 CAATGGGCGT GGATAGCGGT TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT 7201 CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT CCAAAATGTC GTAACAACTC 7261 CGCCCCATTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA TAAGCAGAGC 7321 TCGTTTAGTG AACCGTCAGA TCGCCTGGAG ACGCCATCCA CGCTGTTTTG ACCTCCATAG 7381 AAGACACCGG CGGCCGCATG CAGCGCCGGG GCGCCCTGTT CAGCGTGCCG GGCGGCGGCG 7441 GGAAGATGGC TGCAGGGGAC CTGGGCGAGC TGCTGGTGCC TCATATGCCC ACGATCCGCG 7501 TGCCCAGGTC GGGGGACCGC GTCTACAAGA ACGAGTGCGC CTTCTCCTAC GACTCCCCGA 7561 ACTCTGAAGG TGGGCTCTAC GTATGCATGA ATACCTTTTT GGCCTTTGGA AGGGAACACG 7621 TAGAAAGACA CTTTCGAAAA ACTGGACAGA GCGTATACAT GCACCTGAAG AGGCACATGC 7681 GAGAGAAGGT AAGAGGAGCC TCTGGTGGAG CTTTACCCAA AAGGAGGAAT TCCAAGATAT 7741 TTTTAGATCT AGATATGGAT GACGATTTAA ATAGTGACGA TTACGAATAT GAAGACGAAG 7801 CCAAACTTGT TATATTCCCA GACCACTATG AAATAGCCCT TCCTAACATT GAGGAGTTAC 7861 CAGCCCTGGT AACAATTGCT TGTGATGCAG TGCTCAGCTC AAAGTCCCCT TACAGGAAGC 7921 AGGATCCAGA CACATGGGAA AACGAAGTGC CAGTATCGAA GTATGCCAAC AACCTTGTGC 7981 AACTGGACAA CGGGGTCAGG ATTCCTCCCA GTGGCTGGAA GTGTGCCCGA TGTGACCTGC 8041 GGGAGAACCT CTGGTTGAAT CTGACTGACG GCTCTGTTCT GTGTGGGAAG TGGTTTTTTG 8101 ACAGCTCAGG GGGCAACGGC CACGCACTGG AGCATTACAG GGACATGGGC TATCCTCTGG 8161 CCGTGAAGCT GGGCACCATC ACACCTGATG GGGCAGATGT TTATTCTTTT CAAGAAGAGG 8221 GGCCTGTTTC GGATCCTCAT TTGGCCAAAC ACTTAGCACA TTTTGGGATC GACATGCTCC 8281 ACACGCAAGG GACAGAGAAC GGTCTCCGGG ACAATGACAT CAAACCGAGA GTCAGCGAGT 8341 GGGAAGTGAT CCAGGAGTCA GGAACTAAGC TGAAGCCGAT GTACGGCCCA GGGTACACGG 8401 GCCTGAAGAA CCTGGGCAAC AGTTGCTACC TCAGTTCTGT CATGCAGGCC ATCTTCAGCA 8461 TCCCAGAGTT CCAGAGAGCG TATGTAGGAA ACCTCCCAAG GATATTTGAC TACTCACCGT 8521 TAGATCCAAC GCAGGACTTC AACACACAAA TGACTAAGTT GGGACATGGC CTCCTCTCTG 8581 GCCAGTACTC GAAGCCTCCA GTGAAATCTG AGCTCATTGA ACAGGTGATG AAGGAGGAGC 8641 ACAAGCCTCA GCAGAATGGG ATCTCTCCAC GCATGTTCAA GGCCTTTGTC AGCAAGAGCC 8701 ACCCGGAATT CTCCTCCAAC AGACAGCAGG ATGCCCAGGA GTTTTTCTTG CATTTGGTCA 8761 ATCTGGTAGA GAGGAATCGC ATTGGCTCAG AAAACCCAAG TGATGTTTTC CGGTTTTTGG 8821 TGGAGGAGCG AATTCAATGC TGTCAGACCA GAAAGGTTCG CTACACGGAG AGGGTGGACT 8881 ACCTAATGCA GTTACCTGTG GCCATGGAGG CAGCAACCAA CAAAGATGAG CTGATCACCT 8941 ATGAACTCAT GCGGAGGGAA GCAGAAGCCA ACAGAAGACC CCTACCTGAG CTGGTGCGAG 9001 CCAAGATCCC ATTCAGTGCC TGCCTTCAGG CCTTTGCTGA ACCAGACAAT GTGGATGATT 9061 TCTGGAGCAG TGCTCTGCAG GCCAAGTCTG CAGGGGTCAA AACTTCTCGC TTTGCCTCAT 9121 TCCCTGAATA CTTGGTAGTG CAGATAAAGA AGTTCACTTT TGGTCTTGAC TGGGTTCCCA 9181 GAAAATTTGA TGTTTCTATT GATATGCCAG ACCTACTAGA TATCAGCCAT CTCAGAGCCA 9241 GGGGCTTGCA GCCAGGGGAA GAGGAGCTTC CTGACATCAG CCCCCCCATA GTCATTCCTG 9301 ATGACTCAAA AGACCGCTTG ATGAACCAGT TGATAGACCC CTCAGACATT GATGAGTCTT 9361 CGGTGATGCA GCTGGCTGAG ATGGGCTTCC CTTTGGAAGC CTGCAGGAAG GCTGTGTACT 9421 TCACGGGGAA CACCGGAGCT GAGGTGGCCT TCAACTGGAT TATCGTGCAC ATGGAGGAGC 9481 CTGACTTTGC TGAACCACTG GCCATACCTG GGTATGGAGG GGCTGGGGCC TCTGTCTTTG 9541 GTGCTACTGG ATTGGACAAC CAACCTCCTG AGGAAATCGT AGCTATTATC ACCTCGATGG 9601 GATTCCAGCG AAATCAGGCA GTGCAGGCTC TACAAGCAAC GAATCATAAC CTGGAAAGAG 9661 CACTGGACTG GATCTTCAGC CACCCCGAGT TTGAAGAGGA CAGTGACTTT GTGATCGAGA 9721 TGGAGAACAA TGCAAATGCC AACATCGTGT CTGAGGCCAA GCCAGAGGGA CCCAGAGTGA 9781 AGGATGGGTC TGGAATGTAC GAGTTGTTTG CTTTCATCAG TCACATGGGA ACATCTACAA 9841 TGAGTGGCCA TTATGTTTGC CATATCAAGA AAGAGGGACG ATGGGTGATC TACAATGACC 9901 ACAAAGTTTG TGCCTCAGAA AGGCCCCCCA AAGACCTGGG CTATATGTAC TTTTACCGCA 9961 GGATACCAAG CGACTACAAG GACGACGATG ACAAGTAACT CGAGAATTCT ACCGGGTAGG 10021 GGAGGCGCTT TTCCCAAGGC AGTCTGGAGC ATGCGCTTTA GCAGCCCCGC TGGGCACTTG 10081 GCGCTACACA AGTGGCCTCT GGCCTCGCAC ACATTCCACA TCCACCGGTA GGCGCCAACC 10141 GGCTCCGTTC TTTGGTGGCC CCTTCGCGCC

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.