pCMV-HIVEP3-3×FLAG-Neo plasmid

pCMV-HIVEP3-3×FLAG-Neo plasmid

PVT86275

pCMV-HIVEP3-3×FLAG-Neo plasmid Information

Plasmid name: pCMV-HIVEP3(human)-3×FLAG-Neo
Alias: NM_024503.5;KBP-1; KBP1; KRC; Schnurri-3; SHN3; ZAS3;ZNF40C;
Gene lenght: 7218bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone: pCMV-T7-MCS-3×FLAG-WPRE-Neo
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-HIVEP3-3×FLAG-Neo plasmid Description

pCMV-HIVEP3-3×FLAG-Neo This plasmid is suitable for overexpression in mammalian cells. The CMV enhancer and CMV promoter carried by the plasmid can jointly initiate the efficient expression of the human HIVEP3 gene, promoting the C-terminal fusion expression of the HIVEP3 gene with a 3×FLAG tag, facilitating detection, localization, and purification of the expressed fusion protein using FLAG antibodies. The WPRE protein enhances the processing and stability of the target gene after transcription, helping to increase the mRNA half-life and translation efficiency. The plasmid carries a Neo/G418 resistance gene for selecting host cells that express the target gene.

pCMV-HIVEP3-3×FLAG-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT 661 TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCGTTTA 721 AACTTAAGCT TGGTACCGAG CTCGAATTCG CCACCATGGA TCCTGAACAA AGTGTCAAGG 781 GCACCAAGAA GGCTGAGGGA AGTCCCCGGA AGCGGCTGAC CAAAGGAGAG GCCATTCAGA 841 CCAGTGTTTC TTCCAGCGTC CCATACCCAG GCAGCGGCAC AGCTGCCACC CAAGAGAGCC 901 CCGCCCAAGA GCTCTTAGCC CCGCAGCCCT TCCCGGGCCC CTCATCAGTT CTTAGGGAAG 961 GCTCTCAGGA GAAAACGGGC CAGCAGCAGA AGCCCCCCAA AAGGCCCCCC ATCGAAGCAT 1021 CCGTCCACAT CTCACAGCTT CCGCAGCACC CTCTGACACC AGCATTCATG TCGCCTGGCA 1081 AACCTGAGCA TCTCCTGGAG GGGTCCACAT GGCAACTGGT TGACCCCATG AGACCTGGAC 1141 CCTCTGGCTC CTTCGTGGCC CCTGGGCTCC ATCCTCAGAG CCAGCTCCTT CCTTCCCACG 1201 CTTCCATCAT TCCCCCCGAG GACCTTCCTG GAGTCCCCAA AGTCTTCGTG CCTCGTCCTT 1261 CCCAGGTCTC CTTGAAGCCC ACAGAAGAGG CACACAAGAA GGAGAGGAAG CCCCAGAAGC 1321 CAGGCAAGTA CATCTGCCAG TACTGCAGCC GGCCCTGTGC CAAGCCCAGC GTGCTCCAGA 1381 AGCACATTCG CTCACACACA GGTGAGAGGC CCTACCCCTG CGGCCCCTGT GGCTTCTCCT 1441 TCAAGACCAA GAGTAATCTC TACAAGCACA GGAAGTCCCA TGCCCACCGC ATCAAAGCAG 1501 GCCTGGCCTC AGGCATGGGT GGCGAGATGT ACCCACATGG GCTGGAGATG GAGCGGATCC 1561 CTGGGGAAGA GTTTGAGGAG CCCACTGAGG GAGAAAGCAC AGATTCTGAA GAGGAGACTA 1621 GTGCCACCTC TGGTCACCCT GCAGAGCTCT CCCCAAGACC CAAGCAGCCC CTTCTCTCCA 1681 GCGGGCTATA CAGCTCTGGG AGCCACAGTT CCAGCCACGA ACGCTGTTCC CTGTCCCAGT 1741 CCAGCACAGC CCAGTCACTC GAAGACCCCC CTCCATTTGT GGAACCCTCA TCTGAGCACC 1801 CCCTGAGCCA TAAACCTGAA GACACCCACA CGATTAAGCA GAAGCTGGCC CTCCGCTTAA 1861 GCGAGAGGAA GAAGGTGATC GATGAGCAGG CGTTTCTGAG CCCAGGCAGC AAAGGGAGTA 1921 CTGAGTCTGG GTATTTCTCT CGCTCCGAGA GTGCAGAGCA GCAGGTCAGC CCCCCAAACA 1981 CCAACGCCAA GTCCTACGCT GAGATCATCT TTGGCAAGTG TGGGCGAATA GGACAGCGGA 2041 CCGCCATGCT GACAGCCACC TCCACCCAGC CCCTCCTGCC CCTGTCCACC GAAGACAAGC 2101 CCAGCCTGGT GCCTTTGTCT GTACCCCGGA CGCAGGTGAT CGAGCACATC ACGAAGCTCA 2161 TCACCATCAA CGAGGCCGTG GTGGACACCA GCGAGATCGA CAGCGTGAAG CCAAGGCGGA 2221 GCTCACTGTC CAGGCGCAGC AGCATGGAGT CCCCAAAATC CAGCCTCTAC CGGGAGCCCC 2281 TGTCATCCCA CAGTGAGAAA ACCAAGCCTG AACAATCACT GCTGAGCCTC CAGCACCCGC 2341 CCAGTACCGC CCCCCCTGTG CCTCTCCTGA GAAGCCACTC AATGCCTTCT GCCGCCTGCA 2401 CTATCAGCAC CCCCCACCAC CCCTTCCGAG GTAGCTACTC CTTCGATGAC CATATCACCG 2461 ACTCCGAAGC CCTGAGCCAC AGCAGTCACG TGTTTACCTC CCACCCCCGG ATGCTGAAGC 2521 GCCAGCCGGC AATCGAATTA CCTTTGGGAG GGGAATACAG TTCTGAGGAG CCTGGCCCAA 2581 GCAGCAAAGA CACAGCCTCC AAGCCCTCGG ACGAAGTGGA ACCCAAGGAA AGCGAGCTTA 2641 CCAAAAAGAC CAAGAAGGGT TTGAAAACAA AAGGGGTGAT CTACGAATGT AACATATGTG 2701 GTGCTCGGTA CAAGAAAAGG GATAACTACG AAGCCCACAA AAAATACTAC TGCTCAGAGC 2761 TTCAGATCGC AAAGCCCATC TCTGCAGGCA CCCACACATC TCCAGAAGCT GAAAAGAGTC 2821 AGATTGAGCA TGAGCCGTGG TCCCAAATGA TGCATTACAA ACTGGGAACC ACCCTGGAAC 2881 TCACTCCACT GAGGAAGAGG AGGAAAGAGA AGAGCCTTGG GGACGAGGAA GAGCCACCTG 2941 CCTTTGAGTC CACAAAAAGT CAGTTTGGCA GCCCCGGGCC ATCTGATGCT GCTCGGAACC 3001 TTCCCCTGGA GTCCACCAAG TCACCAGCAG AACCAAGTAA ATCAGTGCCC TCCTTGGAGG 3061 GACCCACGGG CTTCCAGCCA AGGACTCCCA AGCCAGGGTC CGGTTCAGAA TCAGGGAAGG 3121 AGAGGAGAAC AACGTCCAAA GAAATTTCTG TCATCCAGCA CACCAGCTCC TTTGAGAAAT 3181 CTGATTCTCT CGAGCAGCCG AGTGGCTTGG AAGGGGAAGA CAAACCTCTG GCCCAGTTCC 3241 CATCACCCCC ACCTGCCCCA CACGGACGCT CTGCTCACTC CCTGCAGCCT AAGTTGGTCC 3301 GCCAGCCCAA CATTCAGGTT CCTGAGATCC TAGTAACTGA GGAGCCTGAC CGGCCGGACA 3361 CAGAGCCAGA GCCGCCCCCT AAGGAACCTG AGAAGACTGA GGAGTTCCAA TGGCCCCAGC 3421 GCAGCCAGAC ACTTGCCCAG CTCCCAGCTG AGAAGCTGCC ACCCAAAAAG AAGAGGTTGC 3481 GCCTGGCAGA GATGGCCCAA TCATCAGGGG AGTCCAGCTT CGAGTCCTCT GTGCCTCTGT 3541 CTCGCAGCCC GAGCCAGGAA AGCAATGTCT CTTTGAGTGG GTCCAGCCGC TCAGCCTCGT 3601 TTGAGAGGGA TGACCATGGG AAAGCCGAGG CCCCCAGTCC CTCATCTGAC ATGCGCCCCA 3661 AACCCCTGGG CACCCACATG TTGACTGTCC CCAGCCACCA CCCACATGCC CGAGAGATGC 3721 GGAGGTCAGC CTCAGAGCAG AGCCCCAACG TTTCCCATTC TGCCCACATG ACCGAGACAC 3781 GCAGCAAATC CTTTGACTAT GGCAGCTTGT CCTTGACAGG CCCTTCTGCT CCAGCCCCAG 3841 TGGCTCCACC AGCGCGGGTG GCCCCGCCAG AGAGAAGAAA ATGCTTCTTG GTGAGACAGG 3901 CCTCTCTGAG CAGGCCTCCA GAATCTGAGT TGGAGGTTGC CCCCAAGGGA AGACAGGAGA 3961 GCGAAGAACC ACAGCCCTCA TCCAGTAAAC CCTCTGCCAA AAGCTCATTG TCCCAGATTT 4021 CCTCTGCGGC CACCTCACAT GGTGGACCCC CGGGAGGCAA GGGCCCAGGG CAGGACAGGC 4081 CCCCATTGGG GCCCACTGTG CCCTACACAG AAGCACTGCA AGTGTTCCAC CACCCCGTTG 4141 CCCAGACACC CCTGCATGAG AAGCCATACC TGCCCCCACC AGTCTCCCTT TTCTCCTTCC 4201 AGCATCTCGT GCAGCATGAG CCAGGACAGT CTCCAGAATT CTTCTCCACC CAGGCCATGT 4261 CCAGCCTCCT GTCCTCACCA TACTCCATGC CCCCACTTCC TCCCTCCTTA TTTCAAGCCC 4321 CACCGCTTCC TCTCCAGCCT ACTGTTCTGC ACCCAGGCCA ACTCCATCTC CCCCAGCTCA 4381 TGCCTCACCC AGCCAACATC CCCTTCAGGC AGCCCCCTTC CTTCCTCCCC ATGCCATACC 4441 CGACCTCCTC AGCACTGTCT TCTGGGTTTT TCCTGCCTCT GCAATCCCAG TTTGCACTTC 4501 AGCTCCCTGG TGATGTGGAA AGCCATCTGC CCCAGATCAA AACCAGCCTG GCCCCACTGG 4561 CAACAGGAAG TGCTGGCCTC TCCCCCAGCA CAGAGTACAG CAGTGACATC CGGCTACCCC 4621 CTGTGGCTCC CCCAGCCAGC TCCTCAGCAC CTACATCAGC TCCTCCACTG GCCCTGCCTG 4681 CCTGTCCAGA CACCATGGTG TCCCTGGTTG TGCCTGTCCG TGTTCAGACC AATATGCCGT 4741 CCTATGGGAG CGCAATGTAC ACCACCCTTT CCCAGATCTT GGTCACCCAG TCCCAAGGCA 4801 GCTCAGCAAC TGTGGCACTT CCCAAGTTTG AGGAACCCCC ATCAAAGGGG ACGACTGTAT 4861 GTGGTGCAGA TGTGCATGAG GTTGGGCCCG GCCCTTCTGG GTTAAGTGAA GAGCAAAGCA 4921 GAGCTTTCCC AACTCCATAC CTGAGAGTGC CTGTGACATT ACCTGAAAGA AAAGGCACTT 4981 CCCTGTCATC AGAGAGTATC TTGAGCCTGG AGGGGAGTTC ATCAACAGCA GGGGGAAGCA 5041 AACGTGTCCT TTCACCAGCT GGCAGCCTTG AACTTACCAT GGAAACCCAG CAGCAAAAAA 5101 GAGTGAAGGA GGAGGAGGCT TCCAAGGCAG ATGAAAAACT TGAGCTGGTA AAACCATGCA 5161 GTGTGGTCCT TACCAGCACC GAGGATGGGA AGAGGCCAGA GAAATCCCAC TTAGGCAACC 5221 AGGGCCAAGG CAGGAGGGAG CTAGAAATGC TGTCCAGCCT GTCCTCAGAT CCATCTGACA 5281 CAAAGGAAAT TCCTCCCCTC CCTCACCCTG CATTGTCCCA TGGGACAGCC CCAGGCTCAG 5341 AAGCTTTGAA GGAATATCCC CAGCCATCTG GCAAACCTCA CCGAAGAGGG TTGACCCCAC 5401 TGAGCGTGAA GAAAGAAGAT TCCAAGGAAC AACCTGATCT CCCCTCCTTG GCACCTCCGA 5461 GCTCTCTGCC TCTGTCAGAA ACGTCCTCCA GACCAGCCAA GTCACAAGAA GGTACGGACT 5521 CAAAGAAGGT ACTGCAGTTC CCCAGCCTCC ACACAACCAC TAATGTCAGT TGGTGCTATT 5581 TAAACTACAT TAAGCCAAAT CACATCCAGC ATGCAGATAG GAGGTCCTCT GTTTACGCTG 5641 GTTGGTGCAT AAGTTTGTAC AACCCCAACC TTCCGGGGGT TTCCACTAAA GCTGCTTTGT 5701 CCCTCCTGAG GTCTAAGCAG AAAGTGAGCA AAGAGACATA CACCATGGCC ACAGCTCCGC 5761 ATCCTGAGGC AGGAAGGCTT GTGCCATCCA GCTCCCGCAA GCCCCGCATG ACAGAGGTTC 5821 ACCTCCCTTC ACTGGTTTCC CCGGAAGGCC AGAAAGATCT AGCTAGAGTG GAGAAGGAAG 5881 AAGAGAGGAG AGGGGAGCCG GAGGAGGATG CTCCTGCCTC CCAGAGAGGG GAGCCGGCGA 5941 GGATCAAAAT CTTCGAAGGA GGGTACAAAT CAAACGAAGA GTATGTATAT GTGCGAGGCC 6001 GCGGCCGAGG GAAATATGTT TGTGAGGAGT GTGGAATTCG CTGCAAGAAG CCCAGCATGC 6061 TGAAGAAACA CATCCGCACC CACACTGACG TCCGGCCCTA TGTGTGCAAG CACTGTCACT 6121 TTGCTTTTAA AACCAAAGGG AATCTGACTA AGCACATGAA GTCGAAGGCC CACAGCAAAA 6181 AGTGCCAAGA GACAGGGGTG CTGGAGGAGC TGGAAGCCGA AGAAGGAACC AGTGACGACC 6241 TGTTCCAGGA CTCGGAAGGA CGAGAGGGTT CAGAGGCTGT GGAGGAGCAC CAGTTTTCGG 6301 ACCTGGAGGA CTCGGACTCA GACTCAGACC TGGACGAAGA CGAGGATGAG GATGAGGAGG 6361 AGAGCCAGGA TGAGCTGTCC AGACCATCCT CAGAGGCGCC CCCGCCTGGC CCACCACATG 6421 CACTGCGGGC AGACTCCTCA CCCATCCTGG GCCCTCAGCC CCCAGATGCC CCCGCCTCTG 6481 GCACGGAGGC TACACGAGGC AGCTCGGTCT CGGAAGCTGA GCGCCTGACA GCCAGCAGCT 6541 GCTCCATGTC CAGCCAGAGC ATGCCGGGCC TCCCCTGGCT GGGACCGGCC CCTCTGGGCT 6601 CTGTGGAGAA AGACACAGGC TCAGCCTTGA GCTACAAGCC TGTGTCCCCA AGAAGACCGT 6661 GGTCCCCAAG CAAAGAAGCA GGCAGCCGTC CACCACTAGC CCGCAAACAC TCGCTAACCA 6721 AAAACGACTC ATCTCCCCAG CGATGCTCCC CGGCCCGAGA ACCACAGGCC TCAGCCCCAA 6781 GCCCACCTGG CCTGCACGTG GACCCAGGAA GGGGCATGGG CGCTCTCCCT TGTGGGTCTC 6841 CAAGACTTCA GCTGTCTCCT CTCACCCTCT GCCCCCTGGG AAGAGAACTG GCCCCTCGAG 6901 CACATGTGCT CTCCAAACTC GAGGGTACCA CCGACCCAGG CCTCCCCAGA TACTCGCCCA 6961 CCAGGAGATG GTCTCCAGGT CAGGCCGAGT CACCACCACG GTCAGCGCCG CCAGGGAAGT 7021 GGGCCTTGGC TGGGCCGGGC AGCCCCTCAG CGGGGGAGCA TGGCCCAGGC TTGGGGCTGG 7081 ACCCACGGGT TCTCTTCCCG CCCGCGCCTC TACCTCACAA GCTCCTCAGC AGAAGCCCAG 7141 AGACCTGCGC CTCCCCGTGG CAGAAGGCCG AGTCCCGAAG TCCCTCCTGC TCACCCGGCC 7201 CTGCTCATCC TCTCTCCTCC CGACCCTTCT CCGCCCTCCA TGACTTCCAC GGCCACATCC 7261 TGGCCCGGAC AGAGGAGAAC ATCTTCAGCC ACCTGCCTCT GCACTCCCAG CACTTGACCC 7321 GTGCCCCATG TCCCTTGATT CCCATCGGTG GGATCCAGAT GGTGCAGGCC CGGCCAGGAG 7381 CCCACCCCAC CCTGCTGCCA GGGCCCACCG CAGCCTGGGT CAGTGGCTTC TCCGGGGGTG 7441 GCAGCGACCT GACAGGGGCC CGGGAGGCCC AGGAGCGAGG CCGCTGGAGT CCCACTGAGA 7501 GCTCGTCAGC CTCCGTGTCG CCTGTGGCTA AGGTCTCCAA ATTCACACTC TCCTCAGAGC 7561 TGGAGGGCGG GGACTACCCC AAGGAGAGGG AGAGGACCGG CGGAGGCCCG GGCAGGCCTC 7621 CTGACTGGAC ACCCCATGGG ACCGGGGCAC CTGCAGAGCC CACACCCACG CACAGCCCCT 7681 GCACCCCACC CGACACCTTG CCCCGGCCGC CCCAGGGACG CCGGGCAGCG CAGTCCTGGA 7741 GCCCCCGCTT GGAGTCCCCG CGTGCACCGA CCAACCCCGA GCCTTCTGCC ACCCCGCCGC 7801 TGGACCGCAG CAGCTCTGTG GGCTGCCTGG CAGAGGCCTC TGCCCGCTTC CCAGCCCGGA 7861 CGAGGAACCT CTCCGGGGAA CCCAGGACCA GGCAGGACTC CCCCAAGCCC TCAGGAAGTG 7921 GGGAGCCCAG GGCACATCCA CATCAGCCTG AGGACAGGGT TCCCCCCAAC GCTGGATCCG 7981 ACTACAAAGA CCATGACGGT GATTATAAAG ATCATGACAT CGACTACAAG GATGACGATG 8041 ACAAGTAGTG AGGGCCCGAT ATCTCGACAA TCAACCTCTG GATTACAAAA TTTGTGAAAG 8101 ATTGACTGGT ATTCTTAACT ATGTTGCTCC TTTTACGCTA TGTGGATACG CTGCTTTAAT 8161 GCCTTTGTAT CATGCTATTG CTTCCCGTAT GGCTTTCATT TTCTCCTCCT TGTATAAATC 8221 CTGGTTGCTG TCTCTTTATG AGGAGTTGTG GCCCGTTGTC AGGCAACGTG GCGTGGTGTG 8281 CACTGTGTTT GCTGACGCAA CCCCCACTGG TTGGGGCATT GCCACCACCT GTCAGCTCCT 8341 TTCCGGGACT TTCGCTTTCC CCCTCCCTAT TGCCACGGCG GAACTCATCG CCGCCTGCCT 8401 TGCCCGCTGC TGGACAGGGG CTCGGCTGTT GGGCACTGAC AATTCCGTGG TGTTGTCGGG 8461 GAAGCTGACG TCCTTTCCAT GGCTGCTCGC CTGTGTTGCC ACCTGGATTC TGCGCGGGAC 8521 GTCCTTCTGC TACGTCCCTT CGGCCCTCAA TCCAGCGGAC CTTCCTTCCC GCGGCCTGCT 8581 GCCGGCTCTG CGGCCTCTTC CGCGTCTTCG CCTTCGCCCT CAGACGAGTC GGATCTCCCT 8641 TTGGGCCGCC TCCCCGCCTG GAAACGGGGG AGGCTAACTG AAACACGGAA GGAGACAATA 8701 CCGGAAGGAA CCCGCGCTAT GACGGCAATA AAAAGACAGA ATAAAACGCA CGGGTGTTGG 8761 GTCGTTTGTT CATAAACGCG GGGTTCGGTC CCAGGGCTGG CACTCTGTCG ATACCCCACC 8821 GAGACCCCAT TGGGGCCAAT ACGCCCGCGT TTCTTCCTTT TCCCCACCCC ACCCCCCAAG 8881 TTCGGGTGAA GGCCCAGGGC TCGCAGCCAA CGTCGGGGCG GCAGGCCCTG CCATAGCAGA 8941 TCTGCGCAGC TGGGGCTCTA GGGGGTATCC CCACGCGCCC TGTAGCGGCG CATTAAGCGC 9001 GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC 9061 TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT 9121 AAATCGGGGC ATCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA 9181 ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC 9241 TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT 9301 CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGGGGATTT CGGCCTATTG 9361 GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT TAATTCTGTG GAATGTGTGT 9421 CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT 9481 CTCAATTAGT CAGCAACCAG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG 9541 CAAAGCATGC ATCTCAATTA GTCAGCAACC ATAGTCCCGC CCCTAACTCC GCCCATCCCG 9601 CCCCTAACTC CGCCCAGTTC CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT 9661 TATGCAGAGG CCGAGGCCGC CTCTGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT 9721 TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT 9781 GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT 9841 TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC 9901 TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG 9961 ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG 10021 GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC 10081 TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC 10141 GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC 10201 TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC 10261 GGTCTTGTCG ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG 10321 TTCGCCAGGC TCAAGGCGCG CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT 10381 GCCTGCTTGC CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC 10441 CGGCTGGGTG TGGCGGACCG CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA 10501 GAGCTTGGCG GCGAATGGGC TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT 10561 TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT GACGAGTTCT TCTGAGCGGG ACTCTGGGGT 10621 TCGCGAAATG ACCGACCAAG CGACGCCCAA CCTGCCATCA CGAGATTTCG ATTCCACCGC 10681 CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT 10741 CCAGCGCGGG GATCTCATGC TGGAGTTCTT CGCCCACCCC AACTTGTTTA TTGCAGCTTA 10801 TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT 10861 GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT GTATACCGTC 10921 GACCTCTAGC TAGAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA 10981 TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC 11041 CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG 11101 AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG 11161 TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG 11221 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 11281 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 11341 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 11401 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 11461 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 11521 CCCTTCGGGA AGCGTGGCGC TTTCTCAATG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 11581 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 11641 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 11701 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 11761 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGGACAGTA TTTGGTATCT GCGCTCTGCT 11821 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 11881 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 11941 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 12001 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 12061 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG 12121 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG 12181 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC 12241 AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC 12301 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA 12361 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC 12421 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG 12481 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC 12541 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT 12601 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG 12661 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC 12721 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG 12781 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT 12841 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG 12901 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG 12961 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT 13021 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC 13081 ATTTCCCCGA AAAGTGCCAC CTGACGTCGA CGGATCGGGA GATCTCCCGA TCCCCTATGG 13141 TCGACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATCT GCTCCCTGCT 13201 TGTGTGTTGG AGGTCGCTGA GTAGTGCGCG AGCAAAATTT AAGCTACAAC AAGGCAAGGC 13261 TTGACCGACA ATTGCATGAA GAATCTGCTT AGG

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.