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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Human B7-H4 / B7S1 / B7x Protein (ECD, Fc Tag), Endotoxin-Free
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
100.00 µg/1.00 mg
| 规格: | 100.00 µg | 产品价格: | ¥3490.0 |
|---|---|---|---|
| 规格: | 1.00 mg | 产品价格: | ¥22670.0 |
蛋白名称:重组人B7-H4 / B7S1 / B7x蛋白(胞外区, Fc标签)
蛋白构建:A DNA sequence encoding the human VTCN1 (Q7Z7D3-1) (Phe29-Ala258) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. ≥ 90 % as determined by SEC-MALS(Routinely tested).
蛋白内毒素:< 0.5 EU per mg protein.
预测N端:Phe 29
蛋白分子量:The recombinant Human VTCN1/Fc is a disulfide-linked homodimer. The reduced monomer comprises 471 amino acids and has a predicted molecular mass of 52.34 kDa. As a result of glycosylation, the band migrates to 70-80 kDa in the SDS-PAGE under reducing conditions. The molecular weight of this protein is around 147.7 kDa verified by SEC-MALS(Routinely tested).
蛋白NP号:Q7Z7D3-1
蛋白氨基酸序列:Phe29-Ala258
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文献和实验的受体是表达于T细胞的ICDS。B细胞表面ICOS-L与T细胞表面ICOS结合,在T细胞依赖的B细胞活化中起重要作用。 ②B7-H4/B7x.与B7家族其他成员有25%左右同源性,组成性表达于B细胞,在某些肿瘤中(如乳腺癌、卵巢癌等)有较高水平的表达。B7―H4/B7x的受体尚不清楚。B7-H4/B7x可抑制T细胞细胞因子的产生,可能参与免疫应答的负调控。
End-label oligo (20-25 mer) 4 µl 5X T4 Kinase Buffer 5 µl [gamma-32P] ATP (7000 Ci/mmol) 10 µl water + oligo (200 ng) 1 µl T4 Kinase 1. 37 deg C for 1 hour. 2. Heat inactivate at 75 deg C for 10 minutes. 3. Add 1 µl
S1 analysis of yeast mRNA using oligonucleotide probes
2.5 microliters 1 M PIPES pH 6.9 1 microliter 0.25 M EDTA 1-2 x 105 cpm kinased oligonucleotide probe H2O for a final volume of 10 microliters/reaction 1X S1 Buffer: 10 ml 10X S1 Buffer
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