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- Sino Biological
- 50709-M02H
- 2025年08月12日
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- 技术资料
- 规格：
100 µg
蛋白名称：Interferon Gamma蛋白, Interferon Gamma protein
蛋白构建：A DNA sequence encoding the mouse IFNG (P01580) (Met 1-Cys 155) was fused with the Fc region of human IgG1 at the C-terminus.
表达宿主：HEK293 Cells
蛋白纯度：> 92 % as determined by SDS-PAGE
蛋白活性：1. Measured in antiviral assays using L929 cells infected with vesicular stomatitisvirus (VSV). The ED50 for this effect is 2.5-15 ng/mL. 2. Measured by its ability to bind with recombinant mouse IFNGR1-His (Cat:50705-M08H) in a functional ELISA.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：His 23
蛋白分子量：The secreted recombinant mouse IFNG/Fc is a disulfide-linked homodimer. The reduced monomer comprises 374 amino acids and has a calculated molecular mass of 42.5 kDa. As a result of glycosylation, the apparent molecular mass of the monomer is approximately 55 kDa in SDS-PAGE under reducing conditions.
蛋白NP号：P01580
蛋白氨基酸序列：Met1-Cys155
蛋白标签：C-human IgG1-Fc
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Thawing Cells - Remove a vial of Passage 2 Primary Mouse Embryo Fibroblasts (PMEF'S), at a concentration of 3 x 106 from liquid nitrogen and thaw quickly at 37oC. - Add the 1ml
THAWING CELLS Remove a vial of Passage 2 Primary Mouse Embryo Fibroblasts (PMEF'S), at a concentration of 3 x 106 from liquid nitrogen and thaw quickly at 37oC. Add the 1ml of cell containing media to 9ml of warmed PMEF media
Interferon- Gene Therapy for the Treatment of Arthritis
Interferons were originally described as antiviral agents by Isaacs and Lindenmann (1 ). From its subtypes, fibroblast interferon (IFN-β) (2 ) was also found to have important cytostatic (3 ,4 ) as well as immunomodulatory functions
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