- ￥620 - 1810
- Sino Biological
- 北京
- 10605-H01H-UE
- 2026年05月15日
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- 详细信息
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- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Human EGF / Epidermal Growth Factor Protein (Fc Tag), Endotoxin-Free
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
100.00 µg/1.00 mg
| 规格： | 100.00 µg | 产品价格： | ￥620.0 |
|---|---|---|---|
| 规格： | 1.00 mg | 产品价格： | ￥1810.0 |
蛋白名称：重组人EGF / Epidermal Growth Factor蛋白(Fc标签)
蛋白构建：A DNA sequence encoding the mature soluble form of human EGF (NP_001954.2) (Asn 971-Arg 1023) was fused with the Fc region of human IgG1 at the N-terminus.
表达宿主：HEK293 Cells
蛋白纯度：≥ 90 % as determined by SDS-PAGE.
蛋白活性：1.Refer to: Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblast cells. The ED50 for this effect is typically 0.3-1.5 ng/mL . 2.Refer to: Immobilized Recombinant Human EGF / Epidermal Growth Factor Protein (Fc Tag) (Cat: 10605-H01H) at 2 μg/mL (100 μL/well) can bind Recombinant Human EGFR / HER1 / ErbB1 Protein (His Tag) (Cat: 10001-H08H) , the EC50 is 140-450 ng/mL .
蛋白内毒素：< 0.5 EU per mg protein.
预测N端：Glu
蛋白分子量：The recombinant human Fc/EGF chimera is a disulfide-linked homodimeric protein. The reduced monomer consists of 290 amino acids and has a calculated molecular mass of 32.87 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhFc/EGF monomer is approximately 37 kDa due to the glycosylation.
蛋白NP号：NP_001954.2
蛋白氨基酸序列：Asn 971-Arg 1023
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文献和实验Regulator of Epidermal Growth Factor Signaling: Sprouty
Sprouty was first discovered through its downregulatory effect on fibroblast growth factor (FGF) receptor pathway during tracheal development. Sprouty expression is also induced by the epidermal growth factor receptor (EGFR) cascade
Epithelial Cell Migration in Response to Epidermal Growth Factor
Epidermal growth factor (EGF) is a ligand for the EGF receptor, a member of the erbB family of receptor tyrosine kinases. Activation of EGF receptor by EGF or other high-affinity ligands often results in increased migration of cells
Semiquantitative immunoblots of membrane protein-epidermal growth factor
Membrane proteins or cytokines are sometimes difficult to isolate and purify. Our group recently concentrated on epidermal growth factor (EGF) protein expression studies. Mature EGF was initially identified from mouse submaxillary gland extract
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