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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Human B7-H4 / B7S1 / B7x Protein (His Tag), Endotoxin-Free
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
100.00 µg/1.00 mg
| 规格: | 100.00 µg | 产品价格: | ¥3490.0 |
|---|---|---|---|
| 规格: | 1.00 mg | 产品价格: | ¥22670.0 |
蛋白名称:重组人B7-H4 / B7S1 / B7x蛋白(His标签)
蛋白构建:A DNA sequence encoding the human VTCN1 (Q7Z7D3-1) (Phe29-Ala258) with a C-terminal polyhistidine tag was expressed.
表达宿主:HEK293 Cells
蛋白纯度:≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. ≥ 90 % as determined by SEC-MALS(Routinely tested).
蛋白活性:Refer to: Immobilized Recombinant Human B7-H4 / B7S1 / B7x Protein (His Tag) (Cat: 10738-H08H) at 1 μg/mL (100 μL/well) can bind Anti-VTCN1(Research Grade FPA-150 Biosimilar), the EC50 is 1.5-5.0 ng/mL .
蛋白内毒素:< 0.5 EU per mg protein.
预测N端:Phe 29
蛋白分子量:The recombinant Human VTCN1 comprises 241 amino acids and has a predicted molecular mass of 26.8 kDa. As a result of glycosylation, the band migrates approximately 40-60 kDa in the SDS-PAGE under reducing conditions. The molecular weight of this protein is around 41.8 kDa verified by SEC-MALS(Routinely tested).
蛋白NP号:Q7Z7D3-1
蛋白氨基酸序列:Phe29-Ala258
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文献和实验人B7-H4 ( B7-H4 )ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和尿液生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 B7-H4 单抗包被于酶标板上,标准品和样品中的 B7-H4与单抗结合,加入生物素化的抗人 B7-H4 ,形成免疫复合物连接在板上,辣根过氧化物酶标记的 Streptavidin 与生物素结合,加入底物工作液显蓝色,最后加终止
B7-H Abnormalities in Melanoma and Clinical Relevance
as well as altered expression of co-stimulatory and coinhibitory signals. Recently, an important role of B7-H1 and B7-H4 in the immune escape of melanoma has been described. High mRNA and/or protein expression levels of these coinhibitory molecules were detected
的受体是表达于T细胞的ICDS。B细胞表面ICOS-L与T细胞表面ICOS结合,在T细胞依赖的B细胞活化中起重要作用。 ②B7-H4/B7x.与B7家族其他成员有25%左右同源性,组成性表达于B细胞,在某些肿瘤中(如乳腺癌、卵巢癌等)有较高水平的表达。B7―H4/B7x的受体尚不清楚。B7-H4/B7x可抑制T细胞细胞因子的产生,可能参与免疫应答的负调控。
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