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- Sino Biological
- 80372-R07H
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 规格：
100 µg
蛋白名称：TM4SF2/TSPAN7蛋白, TM4SF2/TSPAN7 protein
蛋白构建：A DNA sequence encoding the rat TSPAN7 (ABX10436.1) (Arg113-Met213) was expressed with a polyhistidine tag at the N-terminus.
表达宿主：HEK293 Cells
蛋白纯度：> 85 % as determined by SDS-PAGE
蛋白活性：0
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：His
蛋白分子量：The recombinant rat TSPAN7 consists 120 amino acids and predicts a molecular mass of 13.9 kDa.
蛋白NP号：ABX10436.1
蛋白氨基酸序列：Arg113-Met213
蛋白标签：N-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验蛋白质纯化的原理和方法（Protein Purification Principles and Methods）
and Hydrophobic-Interactions •“Weak” interactions are important for structure and function (activity) of the protein ÆIn most cases the purification must be gentle! Before the purification… •Cultivation of bacteria •Cell disruption
These protocols should yield enough cytosol and organelles for 1-200 MT/Organelle motility assays. Solutions and Reagents • Freshly removed or flash frozen rat liver • PBS •Homogenization Buffer •Homogenization Buffer containing 0.5 mM mM
6. Quant-iT™ Protein Quantitation Kit (Cat. no. Q33210) 实验设备 5 mL Culture Tubes 实验步骤 1. Remove liver aseptically. 2. Flash-freeze liver in foil packets using liquid nitrogen. Store
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