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- 详细信息
- 文献和实验
- 技术资料
- 库存：
1
- 英文名：
IGTR Panel v1.0
- 供应商：
纳昂达（南京）
- 保存条件：
-20℃
IGTR Panel v1.0 覆盖人基因组全部免疫球蛋白 (Immunoglobulin, IG) 和 T 细胞受体 (T cell receptor, TR) 编码基因区段，可用于 DNA 水平重组前或重组后 V、D、J、C 片段的富集，也可用于 RNA 水平成熟转录本的富集
产品表现
DNA 捕获表现
图 1. IGTR Panel v1.0 对不同 gDNA 文库的捕获表现。利用 NadPrep® DNA 通用型文库构建试剂盒 (for Illumina®) 搭配 NadPrep® UDI Adapter (for Illumina®) 构建文库，以 IGTR Panel v1.0 完成杂交捕获。测序模式为 Illumina Novaseq 6000，PE150，利用 BWA 比对到参考基因组 hg38，On-target 按照 reads 数计算。 A. 捕获数据比对率和捕获效率；B. 靶区域覆盖度。
注：样本 gDNA_1-3 分别为： Ramos 细胞系 gDNA 、 Jurkat 细胞系 gDNA 和人类男性基因组 DNA (Promega-male, G1471)。
RNA 捕获表现
图 2. IGTR Panel v1.0 对不同 RNA 文库的捕获表现。利用 NadPrep®Total RNA-To-DNA Module 搭配 NadPrep® DNA 通用型文库构建试剂盒 (for Illumina®>) 建库，以 IGTR Panel v1.0 完成杂交捕获。测序模式为 Illumina Novaseq 6000，PE150，salmon 进行分析。
注：样本 RNA_1-3 分别为： Ramos 细胞系总 RNA、 Jurkat 细胞系总 RNA 和外周血白细胞总 RNA (Takara, 636592)。
RNA 捕获转录本覆盖示例
Ramos 细胞
Jurkat 细胞
图 3. IGTR Panel v1.0 对不同细胞 RNA 捕获转录本的覆盖示例。
捕获灵敏度表现
图 4. IGTR Panel v1.0 对 DNA 和 RNA 文库捕获的灵敏度表现。利用 NadPrep® Total RNA-To-DNA Module 搭配 NadPrep® DNA 通用型文库构建试剂盒 (forIllumina®) 建库，以 IGTR Panel v1.0 完成杂交捕获。测序模式为 Illumina Novaseq 6000，PE150。
注： Ramos+Jurkat DNA 以人类男性基因组 DNA (Promega-male, G1471) 梯度稀释；Ramos+Jurkat RNA 以外周血白细胞总 RNA (TAKARA, 636592) 梯度稀释。
基因列表
IG 编码区覆盖
|
IGHV1-2 |
IGHV1-3 |
IGHV1-18 |
IGHV1-24 |
IGHV1-45 |
IGHV1-46 |
IGHV1-58 |
IGHV1-69 |
IGHV1-69-2 |
IGHV1-69D |
|
IGHV1OR15-1 |
IGHV1OR21-1 |
IGHV1OR16-2 |
IGHV1OR15-9 |
IGHV2-5 |
IGHV2-26 |
IGHV2-70 |
IGHV2-70D |
IGHV2OR16-5 |
IGHV3-7 |
|
IGHV3-11 |
IGHV3-13 |
IGHV3-15 |
IGHV3-16 |
IGHV3-20 |
IGHV3-21 |
IGHV3-23 |
IGHV3-25 |
IGHV3-30 |
IGHV3-33 |
|
IGHV3-35 |
IGHV3-38 |
IGHV3-43 |
IGHV3-48 |
IGHV3-49 |
IGHV3-53 |
IGHV3-62 |
IGHV3-64 |
IGHV3-66 |
IGHV3-72 |
|
IGHV3-73 |
IGHV3-74 |
IGHV3-64D |
IGHV3OR16-6 |
IGHV3OR15-7 |
IGHV3OR16-8 |
IGHV3OR16-9 |
IGHV3OR16-10 |
IGHV3OR16-12 |
IGHV3OR16-13 |
|
IGHV3OR16-17 |
IGHV4-4 |
IGHV4-28 |
IGHV4-31 |
IGHV4-34 |
IGHV4-39 |
IGHV4-59 |
IGHV4-61 |
IGHV4OR15-8 |
IGHV5-51 |
|
IGHV5-10-1 |
IGHV6-1 |
IGHV7-81 |
IGHV7-4-1 |
IGHV8-51-1 |
IGHD1-1 |
IGHD1-7 |
IGHD1-14 |
IGHD1-20 |
IGHD1-26 |
|
IGHD1OR15-1A |
IGHD1OR15-1B |
IGHD2-2 |
IGHD2-8 |
IGHD2-15 |
IGHD2-21 |
IGHD2OR15-2A |
IGHD2OR15-2B |
IGHD3-3 |
IGHD3-9 |
|
IGHD3-10 |
IGHD3-16 |
IGHD3-22 |
IGHD3OR15-3A |
IGHD3OR15-3B |
IGHD4-4 |
IGHD4-11 |
IGHD4-17 |
IGHD4-23 |
IGHD4OR15-4A |
|
IGHD4OR15-4B |
IGHD5-5 |
IGHD5-12 |
IGHD5-18 |
IGHD5-24 |
IGHD5OR15-5A |
IGHD5OR15-5B |
IGHD6-6 |
IGHD6-13 |
IGHD6-19 |
|
IGHD6-25 |
IGHD7-27 |
IGHJ1 |
IGHJ2 |
IGHJ3 |
IGHJ4 |
IGHJ5 |
IGHJ6 |
IGHA1 |
IGHA2 |
|
IGHD |
IGHE |
IGHG1 |
IGHG2 |
IGHG3 |
IGHG4 |
IGHGP |
IGHM |
IGKV1-5 |
IGKV1-6 |
|
IGKV1-8 |
IGKV1D-8 |
IGKV1-9 |
IGKV1-12 |
IGKV1D-12 |
IGKV1-13 |
IGKV1D-13 |
IGKV1-16 |
IGKV1D-16 |
IGKV1-17 |
|
IGKV1D-17 |
IGKV1-27 |
IGKV1-33 |
IGKV1D-33 |
IGKV1-37 |
IGKV1D-37 |
IGKV1-39 |
IGKV1D-39 |
IGKV1D-42 |
IGKV1D-43 |
|
IGKV1OR2-108 |
IGKV2-24 |
IGKV2D-24 |
IGKV2D-26 |
IGKV2-28 |
IGKV2D-28 |
IGKV2-29 |
IGKV2D-29 |
IGKV2-30 |
IGKV2D-30 |
|
IGKV2-40 |
IGKV2D-40 |
IGKV3-7 |
IGKV3D-7 |
IGKV3-11 |
IGKV3D-11 |
IGKV3-15 |
IGKV3D-15 |
IGKV3-20 |
IGKV3D-20 |
|
IGKV3OR2-268 |
IGKV4-1 |
IGKV5-2 |
IGKV6-21 |
IGKV6D-21 |
IGKV6D-41 |
IGKJ1 |
IGKJ2 |
IGKJ3 |
IGKJ4 |
|
IGKJ5 |
IGKC |
IGLV1-36 |
IGLV1-40 |
IGLV1-41 |
IGLV1-44 |
IGLV1-47 |
IGLV1-50 |
IGLV1-51 |
IGLV2-8 |
|
IGLV2-11 |
IGLV2-14 |
IGLV2-18 |
IGLV2-23 |
IGLV2-33 |
IGLV3-1 |
IGLV3-9 |
IGLV3-10 |
IGLV3-12 |
IGLV3-16 |
|
IGLV3-19 |
IGLV3-21 |
IGLV3-22 |
IGLV3-25 |
IGLV3-27 |
IGLV3-32 |
IGLV4-3 |
IGLV4-60 |
IGLV4-69 |
IGLV5-37 |
|
IGLV5-45 |
IGLV5-48 |
IGLV5-52 |
IGLV6-57 |
IGLV7-43 |
IGLV7-46 |
IGLV8-61 |
IGLV8OR8-1 |
IGLV9-49 |
IGLV10-54 |
|
IGLV11-55 |
IGLJ1 |
IGLJ2 |
IGLJ3 |
IGLJ4 |
IGLJ5 |
IGLJ6 |
IGLJ7 |
IGLC1 |
IGLC2 |
|
IGLC3 |
IGLC6 |
IGLC7 |
IGLL1 |
IGLL5 |
TR 编码区覆盖
|
TRAV1-1 |
TRAV1-2 |
TRAV2 |
TRAV3 |
TRAV4 |
TRAV5 |
TRAV6 |
TRAV7 |
TRAV8-1 |
TRAV8-2 |
|
TRAV8-3 |
TRAV8-4 |
TRAV8-6 |
TRAV8-7 |
TRAV9-1 |
TRAV9-2 |
TRAV10 |
TRAV12-1 |
TRAV12-2 |
TRAV12-3 |
|
TRAV13-1 |
TRAV13-2 |
TRAV14DV4 |
TRAV16 |
TRAV17 |
TRAV18 |
TRAV19 |
TRAV20 |
TRAV21 |
TRAV22 |
|
TRAV23DV6 |
TRAV24 |
TRAV25 |
TRAV26-1 |
TRAV26-2 |
TRAV27 |
TRAV29DV5 |
TRAV30 |
TRAV34 |
TRAV35 |
|
TRAV36DV7 |
TRAV38-1 |
TRAV38-2DV8 |
TRAV39 |
TRAV40 |
TRAV41 |
TRAJ1 |
TRAJ2 |
TRAJ3 |
TRAJ4 |
|
TRAJ5 |
TRAJ6 |
TRAJ7 |
TRAJ8 |
TRAJ9 |
TRAJ10 |
TRAJ11 |
TRAJ12 |
TRAJ13 |
TRAJ14 |
|
TRAJ15 |
TRAJ16 |
TRAJ17 |
TRAJ18 |
TRAJ19 |
TRAJ20 |
TRAJ21 |
TRAJ22 |
TRAJ23 |
TRAJ24 |
|
TRAJ25 |
TRAJ26 |
TRAJ27 |
TRAJ28 |
TRAJ29 |
TRAJ30 |
TRAJ31 |
TRAJ32 |
TRAJ33 |
TRAJ34 |
|
TRAJ35 |
TRAJ36 |
TRAJ37 |
TRAJ38 |
TRAJ39 |
TRAJ40 |
TRAJ41 |
TRAJ42 |
TRAJ43 |
TRAJ44 |
|
TRAJ45 |
TRAJ46 |
TRAJ47 |
TRAJ48 |
TRAJ49 |
TRAJ50 |
TRAJ52 |
TRAJ53 |
TRAJ54 |
TRAJ56 |
|
TRAJ57 |
TRAJ58 |
TRAJ59 |
TRAJ61 |
TRAC |
TRBV2 |
TRBV3-1 |
TRBV4-1 |
TRBV4-2 |
TRBV5-1 |
|
TRBV5-3 |
TRBV5-4 |
TRBV5-5 |
TRBV5-6 |
TRBV5-7 |
TRBV6-1 |
TRBV6-2 |
TRBV6-4 |
TRBV6-5 |
TRBV6-6 |
|
TRBV6-7 |
TRBV6-8 |
TRBV7-1 |
TRBV7-2 |
TRBV7-3 |
TRBV7-4 |
TRBV7-6 |
TRBV7-7 |
TRBV7-9 |
TRBV9 |
|
TRBV10-1 |
TRBV10-2 |
TRBV10-3 |
TRBV11-1 |
TRBV11-2 |
TRBV11-3 |
TRBV12-3 |
TRBV12-4 |
TRBV12-5 |
TRBV13 |
|
TRBV14 |
TRBV15 |
TRBV16 |
TRBV17 |
TRBV18 |
TRBV19 |
TRBV20-1 |
TRBV20OR9-2 |
TRBV23-1 |
TRBV23OR9-2 |
|
TRBV24-1 |
TRBV24OR9-2 |
TRBV25-1 |
TRBV27 |
TRBV28 |
TRBV29-1 |
TRBV29OR9-2 |
TRBV30 |
TRBD1 |
TRBD2 |
|
TRBJ1-1 |
TRBJ1-2 |
TRBJ1-3 |
TRBJ1-4 |
TRBJ1-5 |
TRBJ1-6 |
TRBJ2-1 |
TRBJ2-2 |
TRBJ2-3 |
TRBJ2-4 |
|
TRBJ2-5 |
TRBJ2-6 |
TRBJ2-7 |
TRBJ2-2P |
TRBC1 |
TRBC2 |
TRGV1 |
TRGV2 |
TRGV3 |
TRGV4 |
|
TRGV5 |
TRGV8 |
TRGV9 |
TRGV10 |
TRGV11 |
TRGJ1 |
TRGJ2 |
TRGJP1 |
TRGJP2 |
TRGJP |
|
TRGC1 |
TRGC2 |
TRDV1 |
TRDV2 |
TRDV3 |
TRDD1 |
TRDD2 |
TRDD3 |
TRDJ1 |
TRDJ2 |
|
TRDJ3 |
TRDJ4 |
TRDC |
仅限研究使用，不用于临床诊断。
| 产品 | 货号 |
| IGTR Panel v1.0, 16 rxn | 1001942 |
| IGTR Panel v1.0, 96 rxn | 1001941 |
本产品仅限研究使用，不用于临床诊断。
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文献和实验taken directly from this presentation from ABI’s website.Sequencing on the SOLiD machine starts with library preparation. In the simplest fragment library, two different adapters are ligated to sheared genomic DNA (left panel of Fig. 1). If more rigorous
drift particularly in cells known to have an unstable karyotype (i.e. CHO Prod. No. 85050302-1v1, BHK 21 Prod. No. 85011433-1v1) *Loss of cell line due to exceeding finite life-span e.g. human diploid cells such as MRC-5 (Prod. No. 84101801-1v1
are not what they are reported to be then work can be invalidated and resources wasted. There is now considerable evidence of gross cross-contamination of cell lines, in particular with HeLa (Prod. No. 93021013-1v1) where up to 16 lines was offered to ECACC with DNA profiles
