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文献和实验Dual- and Triple-Color Fluorospot
labeled with green fluorophore emitting light at the same wavelength as FITC, streptavidin (SA) labeled with Cy3, and if three cytokines are analyzed, a mouse anti-tag mAb labeled with Cy5 (Mabtech). 5. Mouse mAb anti-human CD28 at 0.1 mg/ml
Anti-GFP/BrdU In Situ Immunostaining
of 2 N HCl x30'. Wash 3x in PBS. Block in 1 mL 10% Horse serum, x30' at room temp. Wash 1x in PBS. Add 0.3 mL Biotinylated anti-BrdU (1:300, with 1% horse serum in PBS). Incubate at 4ºC o.n. or 2 hr. at room temp. Wash 3x in PBS.
Direct Attachment of Conjugate Groups to the 5′ Terminus of Oligodeoxyribonucleotides
Asseline, U., Thuong, N.T., and Hélène, C. 1986. Oligothymidylates substitutés en position 3′ par un dérivè de l' acridine. Nucleosides Nucleotides 5:45‐63.
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