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文献和实验Solutions for Southern Hybridization and Development Hybridization
; sol'n remains turbid Store at 4° C. Add NaAzide for long term storage. Buffer #3: 100mM Tris-Cl, pH 9.5 100mM NaCl 50mM MgCl2 Antibody: 10 µl anti-digoxigenin-AP Fab fragments (Boehringer-Mann cat#1093274) 40ml of Buffer #1 10ml
Southern Blotting: DNA Transfer
mM MgCl2 Antibody: 10 µl anti-digoxigenin-AP Fab fragments (Boehringer-Mann cat#1093274) 40ml of Buffer #1 10ml of Buffer #2 This will last several months of reuse if stored at 4℃.CSPD Ready-to-Use: Keep reused portions separate from used. Store
Southern Blotting(Fred Hutchinson Cancer Research Cente
#2: 2% Blocking Reagent in Buffer #1 Heat to 50-70℃; prepare ~60' in advance; sol'n remains turbid Store at 4℃. Add NaAzide for long term storage. Buffer #3: 100mM Tris-Cl, pH 9.5 100mM NaCl 50mM MgCl2 Antibody: 10 %26micro;l anti-digoxigenin-AP Fab
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