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文献和实验conjugate diluted in block solution for 1 hr at RT. You may need to determine the concentration best for your secondary antibody as well. The Cappel anti-Guinea Pig-FITC is best at 1:800-1:1,000. Wash as before. Aspirate most of last wash off the cells
. You may need to determine the concentration best for your secondary antibody as well. The Cappel anti-Guinea Pig-FITC is best at 1:800-1:1,000. Wash as before. Aspirate most of last wash off the cells but do not dry and mount immediatly. Mount by slopping the mount
Yeast IF without Dehydration--酵母免疫荧光（无甲醇脱水方法)
but this is usually sufficient. Do not let the cells dry! Incubate in secondary antibody conjugate diluted in block solution for 1 hr at RT. You may need to determine the concentration best for your secondary antibody as well. The Cappel anti-Guinea Pig-FITC
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