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文献和实验the following into a tube. CARE Formamide is toxic. Component Volume for 125μL hybridisation (μL) Volume for one slide with four arrays 12xMES 10 50 5M Sodium
setting) and collect the mononuclear cell (MNC) (buffy coat) layer. Resuspend and wash MNCs x 3 with Dulbecco's Phosphate Buffered Saline (DPBS) with 10% Fetal Bovine Serum (FBS) x 10 min, 515 g, before plating into two 12-well plates in full EPC growth
.EukaryotesBy contrast to the simple broth cultures of E. coli, the nutrient requirements of even the simplest eukaryotic culture are complex. Refer to Table 12.1 for a comparison of the ingredients of Nutrient Broth and Minimum Essential Media (MEM), a typical
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