Sino Biological Claudin-6重组蛋白 Nanodisc His&FLAG标签

Claudin-6重组蛋白|Recombinant Human CLDN6 Protein-(Nanodisc) (His & FLAG Tag)（产品说明）
蛋白名称：Human CLDN6 Protein-(Nanodisc) (His & FLAG Tag)
蛋白构建：A DNA sequence encoding the human CLDN6 (P56747) (Met1-Thr184) was expressed, with a Flag tag followed by a polyhistidine tag at the C-terminus.
Nanodisc is a versatile tool for studying membrane proteins. Using styrene-maleic acid (SMA) copolymer, membrane proteins can be extracted directly from prokaryotic and eukaryotic expression systems in the absence of detergents to preserve the protein structure and function better. Compared to membrane scaffold proteins (MSPs) nanodiscs, SMA nanodiscs also have the advantage of preserving proteins' nature by maintaining native lipids surrounded without introducing any heterologous proteins, which allows studies of protein structure and functions in a native-like environment.
表达宿主：HEK293 Cells
蛋白纯度：≥ 90 % as determined by SDS-PAGE
蛋白活性：1.Loaded Anti-CLDN6 antibody on ProA Biosensor, can bind Human CLDN6 Protein(Cat：16658-H61H-NA) with an affinity constant of 0.536nM as determined in BLI assay (Sartorius Octet Red384) (Routinely tested). 2.Captured Anti-Human CLDN6 antibody on CM5 Chip via anti-human IgG Fc antibody, can bind Recombinant Human CLDN6-Nanodisc Protein (His & Flag Tag) (Cat：16658-H61H-NA) with an affinity constant of 7.03 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Met 1
蛋白分子量：The recombinant human CLDN6 consists of 202 amino acids and has a predicted molecular mass of 21.87 kDa. The apparent molecular mass of it is approximately 20.91 KDa in SDS-PAGE under reducing conditions.
蛋白NP号：P56747
蛋白氨基酸序列：Met1-Thr184
蛋白标签：C-His-Flag