- ￥4520
- Sino Biological
- 13911-H09E
- 2026年01月26日
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- 详细信息
- 文献和实验
- 技术资料
- 规格：
100 µg
蛋白名称：DYDC2蛋白, DYDC2 protein
蛋白构建：A DNA sequence encoding the mature form of human DYDC2 (Q96IM9) (Thr102-Pro176) was fused with the GST tag at the N-terminus.
表达宿主：E. coli
蛋白纯度：> 93 % as determined by SDS-PAGE
蛋白活性：0
蛋白内毒素：Please contact us for more information.
预测N端：Met
蛋白分子量：The recombinant human DYDC2 /GST chimera consists of 307 amino acids and has a predicted molecular mass of 35.5 kDa. It migrates as an approximately 37 KDa band in SDS-PAGE under reducing conditions.
蛋白NP号：Q96IM9
蛋白氨基酸序列：Thr102-Pro176
蛋白标签：N-GST
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验GST融合蛋白的准备 Preparation of Glutathione-S-Transferase (GST) Fusion Proteins
expressing each construct (GST alone, GST fusion proteins) into individual 5-ml aliquots of LB broth containing appropriate antibiotic selection. Grow overnight at 37°C with shaking. Although variations of GST fusion protein expression vectors
GST融合蛋白的准备 Preparation of Glutathione
Buffers, Solutions, and Reagents Reduced glutathione, 20 mM prepared in 50 mM Tris-HCl (pH 8.0) Isopropyl-β-D-thio-galactoside (IPTG) LB broth, containing appropriate antibiotic selection Lysis buffer: PBS + 1% Triton X-100, supplemented
.Resuspend beads in 320μl of NETN (~550μl final volume)Aliquot 50μl of beads and add up to 200μl of GST lysate.If Incubate at 4℃ with rocking for 30min.Wash 3x with NETN and add lysate containing protein of interest.Incubate 1-2hr at 4℃ with rocking.Wash 3x
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