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24 months from manufacturing at <-20 °C; 1 month, 2 to 8 °C under sterile conditions.
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文献和实验Assessing Fc Glycan Heterogeneity of Therapeutic Recombinant Monoclonal Antibodies Using NP-HPLC
on the function of antibody molecules is also a regulatory requirement. Glycan mapping by NP-HPLC with fluorescence detection is a sensitive and reproducible method. Labeling of released glycans with anthranilic acid (AA) using reductive amination procedure
Antibody Glycans Characterization
Glycosylation is one of the main IgG post-translational modifications and has essential roles in antibody effectors functions, immunogenicity and plasmatic clearance. In this chapter we discuss and provide detailed protocols for IgG
Antibody Precipitation with Protein A or G
#20333) or ImmunoPure® Immobilized Protein G (Pierce product #20398). Primary antibody Immunoprecipitation buffer: 20 mM sodium phosphate, pH 7.5, 500 mM NaCl, 0.1% SDS, 1% NP40, 0.5% sodium deoxycholate and 0.02% sodium
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