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文献和实验Isolation of Microtubules (Bovine Brain)
minutes at 4° C for further clarification. Collect 10 ml of the supernatant and determine the protein content of your sample using the Bradford protein assay (Appendix G ). Use bovine serum albumin or lysozyme for establishing a standard
Culture of BEND Cells (Bovine Endometrial Cells)
Hams F-12 (N6760) Powdered MEM D-valine modification (M7395) Fetal bovine serum (F4135) Horse serum (H1138) Antibiotic-antimycotic (ABAM) (A9909) D-valine (V1255) Insulin (I5500) Preparation 1. Place 1000 ml of double distilled water
Isolation of Microtubules (Bovine Brain)
content of your sample using the Bradford protein assay ( Appendix G ). Use bovine serum albumin or lysozyme for establishing a standard curve. Decant the remainder of the crude supernatant into a chilled beaker, and add an equal
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