- 询价
- 近岸蛋白(Novoprotein)
- 中国
- DA075
- 2025年11月11日
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- 英文名：
FluB NP Antibody (7F9)
FluB NP Antibody (7F9)
Catalog #: DA075
Spcificty: Antibody recognizes Influenza B Nucleocapsid Protein
Isotype: Mouse IgG1
Source: Cultured in vitro under conditions free from animal derived components.
Product buffer solution: PBS, pH7.4.
Shelf life and storage: 24 months from manufacturing at ≤ -20°C. 1 month, 2 to 8 °C under sterile conditions.
Description: The influenza B virus is the leading cause of annual influenza epidemics and occasional influenza pandemics. NP of influenza B virus plays a central role in viral replication. As a structural protein with no intrinsic enzymatic activity, it is the most abundant viral protein in infected cells. Monomeric NP is a 56 kDa protein with a patch of basic residues capable of binding to a single-strand RNA. NP also contains nuclear localization signals (NLS) and nuclear export signals (NES). The Influenza B virus nucleoprotein (NP) is the major protein component of the genomic viral ribonucleoprotein (vRNP) complexes, which are the replication- and transcription-competent units of Influenza viruses. Early during infection, NP mediates import of vRNPs into the host cell nucleus where viral replication and transcription take place; also newly synthesized NP molecules are targeted into the nucleus, enabling coreplicational assembly of progeny vRNPs.
PARAMETERS TESTED ON EACH LOT
Product appearance: Liquid, may turn slightly opaque during storage.
Product concentration: 5.0 mg/ml (+/- 10 %)
Purity: ≥95 %
Bioactivity-Elisa:
Immobilized Influenza B NP (Cat#DRA131) at 2μg/ml (100 μl/well)can bind FluB NP Antibody 7F9(Cat#DA075).The ED50 of FluB NP Antibody 7F9(Cat#DA075) is 15.95 ng/ml .
FOR RESEARCH OR FURTHER MANUFACTURING USE ONLY
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文献和实验Assessing Fc Glycan Heterogeneity of Therapeutic Recombinant Monoclonal Antibodies Using NP-HPLC
on the function of antibody molecules is also a regulatory requirement. Glycan mapping by NP-HPLC with fluorescence detection is a sensitive and reproducible method. Labeling of released glycans with anthranilic acid (AA) using reductive amination procedure
Antibody Glycans Characterization
Glycosylation is one of the main IgG post-translational modifications and has essential roles in antibody effectors functions, immunogenicity and plasmatic clearance. In this chapter we discuss and provide detailed protocols for IgG
Antibody Precipitation with Protein A or G
#20333) or ImmunoPure® Immobilized Protein G (Pierce product #20398). Primary antibody Immunoprecipitation buffer: 20 mM sodium phosphate, pH 7.5, 500 mM NaCl, 0.1% SDS, 1% NP40, 0.5% sodium deoxycholate and 0.02% sodium
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