- ¥1699.90
- 阿拉丁
- 上海
- D128600
- 2026年05月08日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
2-8°C储存；低温运输
- 英文名：
Deoxyribonuclease II from porcine spleen
- 库存：
现货
- 供应商：
上海阿拉丁生化科技股份有限公司
- 规格：
D128600-80KU
基本描述
产品名称：脱氧核糖核酸酶Ⅱ 来源于猪脾
规格或纯度：EnzymoPure™, ≥800 units/mg dry weight
产品介绍：
Deoxyribonuclease II from porcine spleen has a molecular weight of 38 kDa. The enzyme is a glycoprotein endonuclease with dimeric structure. Optimum pH range is 4.5-5.0 at ionic strength 0.15 M. Deoxyribonuclease II (Acid DNase) hydrolyzes deoxyribonucleotide linkages in native and denatured DNA yielding products with 3'-phosphates. It also acts on p-nitrophenylphosphodiesters at pH 5.6-5.9. Bernardi (BBRC, 17, 573, 1971) describes a three stage degradation of native DNA by DNase II.DNase II has been used to treat transformed cells during the purification of β-lactamase. It has also been used for the preparation of adenoma tissue in a study that investigated the effect of somatoprim on growth hormone secretion in human adenoma cell cultures (hSA). Deoxyribonuclease II from bovine spleen has been used in a study that conducted a partial purification of deoxyribonucleases from eggs and liver of Xenopus laevis. Deoxyribonuclease II from bovine spleen has also been used in a study to investigate nucleic acid and protein synthesis of splenic lymphocytes.
级别：EnzymoPure™
产品规格参数
浓度：≥800 units/mg dry weight
储存条件：2-8°C储存
运输条件：低温运输
CAS编号和信息：9025-64-3
酶学委员会编号：I.U.B.: 3.1.22.1
单位定义：One Unit causes an increase in absorbance at 260 nm of 0.001 per minute at 25°C, pH 4.6 using highly polymerized DNA as substrate.
功能和特点
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文献和实验High Molecular Weight Yeast Liquid DNA Preparation
Purpose: To isolate intact, high molecular weight DNA from yeast cells for subcloning and rare cutting restriction enzyme analysis. One can expect a yield of 100-200 � of DNA per prep. Time required: Day
How to Make Simple Solutions and Dilutions
= 200 ul = 0.2 ml C2 = the new concentration = 25 mg/ ml By algebraic rearrangement: V1 = (V2 x C2) / C1 V1 = (0.2 ml x 25 mg/ml) / 100 mg/ml and after cancelling the units, V1 = 0.05 ml, or 50 ul
How to Make Simple Solutions and Dilutions
concentration = 200 ul = 0.2 ml C2 = the new concentration = 25 mg/ ml By algebraic rearrangement: V 1 = (V 2 x C 2 ) / C 1 V 1 = (0.2 ml x 25 mg/ml) / 100 mg/ml and cancel the units
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