- ¥1695.90
- 阿拉丁
- 上海
- D128605
- 2026年05月08日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20°C储存；超低温运输
- 英文名：
Deoxyribonuclease II from porcine spleen(Purified,Solution)
- 库存：
现货
- 供应商：
上海阿拉丁生化科技股份有限公司
- 规格：
D128605-5KU
基本描述
产品名称：脱氧核糖核酸酶Ⅱ 来源于猪脾(纯化,溶液)
规格或纯度：EnzymoPure™, ≥12,000 units/mg protein
产品介绍：
Deoxyribonuclease II from porcine spleen has a molecular weight of 38 kDa. The enzyme is a glycoprotein endonuclease with dimeric structure. Optimum pH range is 4.5-5.0 at ionic strength 0.15 M. Deoxyribonuclease II (Acid DNase) hydrolyzes deoxyribonucleotide linkages in native and denatured DNA yielding products with 3'-phosphates. It also acts on p-nitrophenylphosphodiesters at pH 5.6-5.9. Bernardi (BBRC, 17, 573, 1971) describes a three stage degradation of native DNA by DNase II.DNase II has been used to treat transformed cells during the purification of β-lactamase. It has also been used for the preparation of adenoma tissue in a study that investigated the effect of somatoprim on growth hormone secretion in human adenoma cell cultures (hSA). Deoxyribonuclease II from bovine spleen has been used in a study that conducted a partial purification of deoxyribonucleases from eggs and liver of Xenopus laevis. Deoxyribonuclease II from bovine spleen has also been used in a study to investigate nucleic acid and protein synthesis of splenic lymphocytes.
级别：EnzymoPure™
产品规格参数
浓度：≥12,000 units/mg protein
储存条件：-20°C储存
运输条件：超低温运输
CAS编号和信息：9025-64-3
功能和特点
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文献和实验-2 Digitonin 235 1,229 70,000 - - C12 E8 - 542 65,000 0.005 8.7 x 10-5 Lubrol PX - 582 64,000 0.006 1.0 x 10-4 Triton X-100 - 650 90,000 0.021 3.0 x 10-4 Nonident P-40 - 603 90,000 0.017 3.0 x 10-4 Tween 80
Protocol of the protein extraction method
of the protein. Annotation: the component of buffer: Lysis bufferⅠ (250ml): Glycerd: 25ml (10%) Triton X-100: 2.5ml (1%) Hepes (PH7.5): 12.5ml (50mM) NaCl (150mM): 37.5ml NaF (2mM) EDTA
Purification of Antiserum or Ascites by Protein A/G Chromatography
: Since sodium azide is toxic, wear gloves and handle the stock solution with care.Ⅳ Clarify the antiserum by centrifugation at 15,000 x g for 5 minutes at 4. This step is performed to sediment aggregates of denatured protein and lipid; it is an important step
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