Adapter, Bottle, Conical, 500

Dropout plates for yeast

of a 10x master solution: Grab a 500 ml conical flask Figure out how much of your dropout powder you''re going to need and weigh it out For example, the CSM - TRP mix we have is 0.74g/L, so for a 200ml of a 10x solution

AMNIOTIC FLUID CULTURES ON COVERSLIPS

the appropriate A or B media bottle. Follow these coverslips until ready for a full media change and reset.) H. Change the media by using a sterile pipette to transfer the suspension into a sterile 15 ml conical tube. (All 'A'coverslips in one tube, and all 'B

Transformation of E. coli by Electroporation

for electroporation. 1. Use a fresh colony of DH5α (or other appropriate host strain) to inoculate 5 ml of SOB (without magnesium) medium in a 50 ml sterile conical tube. Grow cells with vigorous aeration overnight at 37EC. 2. Dilute 2.5 ml of cells into 250