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- Beckman Coulter贝克曼库尔特
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- 2025年07月15日
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文献和实验Materials (Solutions are all available from the media room) 200ml bottle of 2x SD 200ml bottle of 4% agar -- make sure to sign it out 40% glucose CSM minus the relevant marker(s), as powder Stack
Transformation of E. coli by Electroporation
for electroporation. 1. Use a fresh colony of DH5α (or other appropriate host strain) to inoculate 5 ml of SOB (without magnesium) medium in a 50 ml sterile conical tube. Grow cells with vigorous aeration overnight at 37EC. 2. Dilute 2.5 ml of cells into 250
AMNIOTIC FLUID CULTURES ON COVERSLIPS
the appropriate A or B media bottle. Follow these coverslips until ready for a full media change and reset.) H. Change the media by using a sterile pipette to transfer the suspension into a sterile 15 ml conical tube. (All 'A'coverslips in one tube, and all 'B
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