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文献和实验Alkaline Lysis and CsCl Purification of BAC DNA for Transgenic Production.
4. Wash cells by resuspending in 25 mls of ICE COLD 10mM NaCl (0.1ml 5M into 50ml final) per 250 ml bottle, pellet at 3800 rpm for 10minutes at 4°C. 5. Decant supernatant, (freezing the pellet at minus 80°C overnight at this step increases cell
Standard Operating Procedures for T1-Phage Testing Assay
, Rossville 50 ml polypropylene tubes, Falcon, Corning Q-tray, Genetix 96-pins stamping tool, V & P Scientific, Inc. Autoclave, refrigerator, incubator, waterbath, and laminar flow hood. III. Procedures: Method
Fibronectin Sigma F0895-2MG Glass Bottle (250ml) with Cap Cole Parmer Instrument Co. 34594
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