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文献和实验Clonality - X Chromosome Inactivation Assay
(1:1:1, v:v:v) (Gibco) 3 M Na Acetate, pH 5.2 (Life Technologies) Isopropanol Glycogen, 10 mg/ml (GenHunter) 70% ethanol Buffer 1 (50 mM Tris-HCl, pH 8.0, 10 mM MgCl2, 50 mM NaCl) Hha I restriction enzyme (Life Technologies) REact 2 Buffer
Purification of Plasmid from 50 ml-culture
of cold 70% ethanol. Discard the fluid. 14. Add to the pellet 400 ul of TE containing DNase-free RNase A (20 ug/ml). Incubate the tube for 30 min at 37 C. 15. After 30 min, carefully check the content of the tube. If nucleic acid pellet
Purification of Plasmid from 50 ml-culture
of the tube with 500 ul of cold 70% ethanol. Discard the fluid. 14. Add to the pellet 400 ul of TE containing DNase-free RNase A (20 ug/ml). Incubate the tube for 30 min at 37 C. 15. After 30 min, carefully check the content of the tube. If nucleic
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